首页> 外文期刊>Artificial cells, blood substitutes, and immobilization biotechnology. >Construction of an Amperometric Ascorbate Biosensor Using Epoxy Resin Membrane Bound Lagenaria siceraria Fruit Ascorbate Oxidase
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Construction of an Amperometric Ascorbate Biosensor Using Epoxy Resin Membrane Bound Lagenaria siceraria Fruit Ascorbate Oxidase

机译:树脂膜结合紫胶洋紫苏果抗坏血酸氧化酶构建抗坏血酸生物传感器

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: Ascorbate oxidase purified from Lagenaria siceraria fruit was immobilized onto epoxy resin “Araldite” membrane with 79.4% retention of initial activity of free enzyme. The biosensor showed optimum response within 15s at pH 5.8 and 35°C, which was directly proportional to ascorbate concentration ranging from 1-100μM. There was a good correlation (R2 = 0.99) between serum ascorbic acid values by standard enzymic colorimetric method and the present method. The enzyme electrode was used for 200 times without considerable loss of activity during the span of 90 days when stored at 4°C.
机译::从紫叶忍冬果实中纯化的抗坏血酸氧化酶固定在环氧树脂“ Araldite”膜上,保留了79.4%的游离酶初始活性。该生物传感器在pH 5.8和35°C下在15秒钟内显示出最佳响应,这与抗坏血酸浓度在1-100μM之间成正比。通过标准酶比色法测定的血清抗坏血酸值与本方法之间具有良好的相关性(R2 = 0.99)。当在4°C下储存90天后,酶电极使用了200次,而活性没有明显损失。

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