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首页> 外文期刊>Asian Journal of Pharmaceutical and Clinical Research >ANTIOXIDANT PROFILE AND PHYTOCHEMICAL CONTENT OF THREE KINDS OF LEMON GRASS GROWN IN WEST JAVA-INDONESIA
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ANTIOXIDANT PROFILE AND PHYTOCHEMICAL CONTENT OF THREE KINDS OF LEMON GRASS GROWN IN WEST JAVA-INDONESIA

机译：西爪哇-印度尼西亚的三种柠檬草的抗氧化剂特性和理化含量

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Objective: The aims of this research were to determine antioxidant activity from various herbs extracts of three kinds of lemon grass using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) and correlation of total phenolic content (TPC), total flavonoid content (TFC), and total carotenoid contents (TCC) with their inhibitory concentration 50% (IC ) of DPPH and exhibitory concentration 50% (EC 50 ) of FRAP. Methods: A sample was extracted by reflux method using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities using DPPH and FRAP assays, determination of TPC, TFC, and TCC were carried out by ultraviolet -visible spectrophotometry and correlation with their IC 50 of DPPH and EC 50 of FRAP capacities were analyzed by Pearson’s method. Results: The ethanolic herbs extract of Cymbopogon citratus (CC) had the lowest IC 50 50 of DPPH scavenging activity 2.75 μg/ml and the lowest EC of FRAP capacity 12.22 μg/ml. Ethanolic herbs extract of Cymbopogon winterianus exposed the highest phenolic content and its n-hexane extract presented the highest carotenoid content. Ethyl acetate herbs extract of CC gave the highest flavonoid content. There was significantly negative correlation between TPC in CC herbs extract with their IC 50 of DPPH and EC 50 of FRAP. Conclusions: All herbs extracts from three kinds of lemon grass were categorized as a very strong antioxidant by DPPH method. Phenolic compounds in CC were the major contributor in antioxidant activities by DPPH and FRAP methods. DPPH and FRAP gave linear result in antioxidant activities of herbs extract of three kinds of lemon grass.
机译：目的：本研究的目的是使用两种抗氧化剂测试方法，即2,2-二苯基-1-吡啶甲基肼基（DPPH）和还原铁抗氧化剂能力（FRAP）来测定三种柠檬草的各种草药提取物的抗氧化剂活性。总酚含量（TPC），总类黄酮含量（TFC）和总类胡萝卜素含量（TCC）与它们对DPPH的抑制浓度50％（IC）和对FRAP的展示浓度50％（EC 50）的相关性。方法：使用不同极性的溶剂通过回流方法提取样品。使用旋转蒸发仪蒸发提取物。用紫外可见分光光度法进行了DPPH和FRAP分析的抗氧化活性，TPC，TFC和TCC的测定，并通过皮尔森法分析了它们与DPPH的IC 50和FRAP容量的EC 50的相关性。结果：柠檬柏（Cymbopogon citratus，CC）的乙醇提取物对DPPH的清除活性最低，IC 50 50为2.75μg/ ml，对FRAP的最低EC为12.22μg/ ml。 Cymbopogon winterianus的乙醇提取物的酚含量最高，其正己烷提取物的类胡萝卜素含量最高。 CC的乙酸乙酯提取物提取出的类黄酮含量最高。 CC草药提取物中的TPC与DPPH的IC 50和FRAP的EC 50显着负相关。结论：DPPH法将三种柠檬草的所有草药提取物归类为非常强的抗氧化剂。通过DPPH和FRAP方法，CC中的酚类化合物是抗氧化活性的主要贡献者。 DPPH和FRAP对三种柠檬草中草药提取物的抗氧化活性呈线性关系。

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