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首页> 外文期刊>Asian Journal of Pharmaceutical and Clinical Research >SCREENING AND IDENTIFICATION OF HEAVY METAL-TOLERANT ENDOPHYTIC FUNGI LASIODIPLODIA THEOBROMAE FROM BOSWELLIA OVALIFOLIOLATA AN ENDEMIC PLANT OF TIRUMALA HILLS
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SCREENING AND IDENTIFICATION OF HEAVY METAL-TOLERANT ENDOPHYTIC FUNGI LASIODIPLODIA THEOBROMAE FROM BOSWELLIA OVALIFOLIOLATA AN ENDEMIC PLANT OF TIRUMALA HILLS

机译：丘陵山地特有植物Boswellia ovalifoliolata的重金属耐内生真菌内生真菌Theobromae的筛选和鉴定

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Aim: The aim of this study was to evaluate the heavy metal resistance potentiality of endophytic fungi isolated from the leaves of Boswellia ovalifoliolata, an endemic medicinal plant of Tirumala Hills. Methods: Initially, isolation of fungal endophytes was carried out. Isolated fungi were screened for the heavy metal resistance against Co, Cd, Cu and Zn using growth and evaluated their maximum tolerant capacity. Molecular identification of endophytic fungi was carried out by 18S rRNA gene amplification and Sanger’s nucleotide sequencing. Phylogenetic tree was constructed using NCBI Clustal W. Results: Ten different endophytic fungi were isolated from the leaves of B. ovalifoliolata. Among the isolated endophytic fungi, five showed resistance to Co, Cd, Cu, and Zn. The most resistant fungus was identified as Lasiodiplodia theobromae based on 18S rRNA gene sequencing. Conclusions: L. theobromae was isolated from B. ovalifoliolata and identified as one of the useful fungi involved in mycoremediation against heavy metal toxicity. Keywords: Heavy metals, Endophytic fungi, Endemic plant, Bioremediation.
机译：目的：本研究的目的是评估从Tirumala Hills的特有药用植物卵叶乳香（Boswellia椭​​圆叶）中分离出的内生真菌对重金属的抵抗能力。方法：最初，进行了真菌内生菌的分离。使用生长筛选分离的真菌对Co，Cd，Cu和Zn的重金属抗性，并评估其最大耐受能力。通过18S rRNA基因扩增和Sanger核苷酸测序对内生真菌进行分子鉴定。用NCBI Clustal W构建系统发生树。结果：从卵圆双歧杆菌的叶子中分离出十种不同的内生真菌。在分离出的内生真菌中，有五个对Co，Cd，Cu和Zn表现出抗性。根据18S rRNA基因测序，最有抗性的真菌被鉴定为桔梗。结论：Theobromae分离自卵形芽孢杆菌，被鉴定为参与抗重金属毒性的mycoremediation的有用真菌之一。关键词：重金属;内生真菌;特有植物;生物修复。

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