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首页> 外文期刊>Asian Journal of Pharmaceutical and Clinical Research >GAS CHROMATOGRAPHY-MASS SPECTROSCOPY STUDIES ON CESTRUM NOCTURNUM MACERATED METHANOLIC EXTRACT
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GAS CHROMATOGRAPHY-MASS SPECTROSCOPY STUDIES ON CESTRUM NOCTURNUM MACERATED METHANOLIC EXTRACT

机译:气相色谱-气相色谱法提取夜精中提取的甲醇提取物

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Objective: Phytochemical screening has revealed the presence of alkaloids, glycosides, carbohydrates, steroids, flavonoids, saponins, and tannins from methanolic extract of Cestrum nocturnum. In this research, we have identified and confirmed the structures of the constituents by gas chromatography– mass spectrometry (GC-MS) from the methanolic extract of C. nocturnum leaves. Methods: Shade-dried leaves were powdered and extracted with methanol by cold extraction procedures and the crude extract obtained was used for GC-MS. Results: There were total five components prominently eluted from C. nocturnum extract, viz., ethyl citrate, phytol, 4-isobutylmorpholine, n-hexadecanoic acid, and α-amyrin. Conclusion: GC-MS studies helped to recognize the phytochemical constituents based on their retention time and verification of MS libraries. In future, C. nocturnum leaves may be subjected to different extraction procedures and number of elucidating phytoconstituents can be studied for their ethnopharmacological relevance citing the literature. Keywords: Cestrum nocturnum, Gas chromatography-mass spectroscopy, α-Amyrin, n-Hexadecanoic acid, Ethyl citrate, Phytol, 4-Isobutylmorpholine.
机译:目的:植物化学筛选已发现夜蛾甲醇提取物中存在生物碱,糖苷,碳水化合物,类固醇,类黄酮,皂角苷和单宁酸。在这项研究中,我们已经通过气相色谱-质谱法(GC-MS)从夜蛾叶片的甲醇提取物中鉴定并确认了成分的结构。方法:将阴凉干燥的叶子打成粉末,用冷提取方法用甲醇提取,所得粗提取物用于GC-MS。结果:从夜曲提取物中突出洗脱的共有五种成分,即柠檬酸乙酯,植醇,4-异丁基吗啉,正十六烷酸和α-淀粉糊精。结论:GC-MS研究有助于根据其保留时间和MS库验证来识别植物化学成分。将来,夜曲叶可能会经历不同的提取程序,并且可以参考文献来研究阐明植物成分的数量,以了解其民族药理学意义。关键词:夜精,气相色谱-质谱,α-香豆素,正十六烷酸,柠檬酸乙酯,植物甾醇,4-异丁基吗啉。

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