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首页> 外文期刊>Annals of laboratory medicine. >Evaluation of Peptide Nucleic Acid Probe-based Real-time PCR for Detection of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria in Respiratory Specimens
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Evaluation of Peptide Nucleic Acid Probe-based Real-time PCR for Detection of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria in Respiratory Specimens

机译：基于肽核酸探针的实时荧光定量PCR检测呼吸道标本中结核分枝杆菌复合体和非结核分枝杆菌的评估

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Background: A peptide nucleic acid (PNA) probe-based real-time PCR (PNAqPCRTM TB/ NTM detection kit; PANAGENE, Korea) assay has been recently developed for the simultaneous detection of Mycobacterium tuberculosis complex (MTBC) and nontuberculous mycobacteria (NTM) in clinical specimens. The study was aimed at evaluation of the performance of PNA probe-based real-time PCR in respiratory specimens. Methods: To evaluate potential cross-reactivity, the extracted DNA specimens from Mycobacterium species and non-mycobacterial species were tested using PNA probe-based real-time PCR assay. A total of 531 respiratory specimens (482 sputum specimens and 49 bronchoalveolar washing fluid specimens) were collected from 230 patients in July and August, 2011. All specimens were analyzed for the detection of mycobacteria by direct smear examination, mycobacterial culture, and PNA probe-based real-time PCR assay. Results: In cross-reactivity tests, no false-positive or false-negative results were evident. When the culture method was used as the gold standard test for comparison, PNA probebased real-time PCR assay for detection of MTBC had a sensitivity and specificity of 96.7% (58/60) and 99.6% (469/471), respectively. Assuming the combination of culture and clinical diagnosis as the standard, the sensitivity and specificity of the new real-time PCR assay for detection of MTBC were 90.6% (58/64) and 99.6% (465/467), respectively. The new real- time PCR for the detection of NTM had a sensitivity and specificity of 69.0% (29/42) and 100% (489/489), respectively. Conclusions: The new real-time PCR assay may be useful for the detection of MTBC in respiratory specimens and for discrimination of NTM from MTBC.
机译：背景：最近开发了一种基于肽核酸（PNA）探针的实时PCR（PNAqPCRTM TB / NTM检测试剂盒;韩国PANAGENE）检测方法，用于同时检测结核分枝杆菌复合体（MTBC）和非结核分枝杆菌（NTM）在临床标本中。这项研究旨在评估基于PNA探针的实时PCR在呼吸道标本中的性能。方法：为了评估潜在的交叉反应性，使用基于PNA探针的实时PCR检测从分枝杆菌属和非分枝杆菌属物种中提取的DNA标本。 2011年7月和8月，共从230例患者中收集了531份呼吸道标本（482份痰标本和49支气管肺泡冲洗液标本）。所有标本均通过直接涂片检查，分枝杆菌培养和PNA探针进行了分析以检测分枝杆菌。基于实时PCR的检测。结果：在交叉反应测试中，没有明显的假阳性或假阴性结果。当将培养方法用作比较的金标准测试时，基于PNA探针的实时PCR检测MTBC的灵敏度和特异性分别为96.7％（58/60）和99.6％（469/471）。以培养和临床诊断相结合为标准，新的实时PCR检测MTBC的灵敏度和特异性分别为90.6％（58/64）和99.6％（465/467）。用于检测NTM的新型实时PCR的灵敏度和特异性分别为69.0％（29/42）和100％（489/489）。结论：新的实时荧光定量PCR检测方法可能对检测呼吸道标本中的MTBC和鉴别MTBC中的NTM有用。

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