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首页> 外文期刊>Atmospheric chemistry and physics >Annual distribution of allergenic fungal spores in atmospheric particulate matter in the Eastern Mediterranean; a comparative study between ergosterol and quantitative PCR analysis
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Annual distribution of allergenic fungal spores in atmospheric particulate matter in the Eastern Mediterranean; a comparative study between ergosterol and quantitative PCR analysis

机译：东地中海大气颗粒物中的过敏性真菌孢子的年度分布;麦角固醇与定量PCR分析的比较研究

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Airborne fungal spores are an important fraction of atmospheric particulatematter and are major causative agents of allergenic and infectious diseases.Predicting the variability and species of allergy-causing fungal sporesrequires detailed and reliable methods for identification andquantification. There are diverse methods for their detection in theatmosphere and in the indoor environments; yet, it is important to optimizesuitable methods for characterization of fungal spores in atmosphericsamples. In this study we sampled and characterized total and specificairborne fungal spores from PM10 samples collected in Rehovot, Israel overan entire year. The total fungal spore concentrations vary throughout theyear although the species variability was nearly the same. Seasonalequivalent spore concentrations analyzed by real-time quantitative-PCR-basedmethods were fall > winter > spring > summer. Reported concentrationsbased on ergosterol analysis for the same samples were and fall > spring > winter > summer. Correlation between the two analytical methods wasfound only for the spring season. These poor associations may be due to theper-spore ergosterol variations that arise from both varying productionrates, as well as molecular degradation of ergosterol. While conversion ofgenome copies to spore concentration is not yet straightforward, thepotential for improving this conversion and the ability of qPCR to identifygroups of fungi or specific species makes this method preferable forenvironmental spore quantification. Identifying tools for establishing therelation between the presence of species and the actual ability to induceallergies is still needed in order to predict the effect on human health.
机译：空气传播的真菌孢子是大气颗粒物的重要组成部分，是致敏性和传染性疾病的主要病原。预测致敏性真菌孢子的变异性和种类需要详细可靠的鉴定和定量方法。在大气层和室内环境中有多种检测方法。然而，重要的是要优化用于表征大气样品中真菌孢子的方法。在这项研究中，我们从以色列Rehovot整年收集的PM 10 样品中取样并鉴定了总的和特定的空气传播的真菌孢子。尽管物种变异性几乎相同，但真菌孢子的总浓度全年仍在变化。通过基于实时定量PCR的方法分析的季节性等效孢子浓度为秋季>冬季>春季>夏季。基于麦角固醇分析的相同样品的报告浓度为秋季>春季>冬季>夏季。仅在春季发现了两种分析方法之间的相关性。这些差的缔合可能是由于生产率变化以及麦角固醇的分子降解引起的全孢子麦角固醇的变化。虽然基因组拷贝到孢子浓度的转化尚不直接，但改善这种转化的潜力以及qPCR鉴定真菌或特定物种的能力使该方法成为环境芽孢定量的首选方法。为了预测对人类健康的影响，仍然需要鉴定工具来建立物种的存在与诱发过敏的实际能力之间的关系。

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