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首页> 外文期刊>Annals of laboratory medicine. >Comparison of the Luminex xTAG Respiratory Viral Panel Fast v2 Assay With Anyplex II RV16 Detection Kit and AdvanSure RV Real-Time RT-PCR Assay for the Detection of Respiratory Viruses
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Comparison of the Luminex xTAG Respiratory Viral Panel Fast v2 Assay With Anyplex II RV16 Detection Kit and AdvanSure RV Real-Time RT-PCR Assay for the Detection of Respiratory Viruses

机译：Luminex xTAG呼吸道病毒快速检测试剂盒与Anyplex II RV16检测试剂盒和AdvanSure RV实时RT-PCR检测试剂盒检测呼吸道病毒的比较

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Background: The accurate and rapid identification of the causative viruses is important for the timely diagnosis and management of respiratory infections. Multiplex molecular diagnostic techniques have been widely adopted to detect respiratory viruses. We compared the results of a newly upgraded, multiplex, molecular bead-based respiratory viral panel (RVP) assay with the results of Anyplex II RV16 detection kit and AdvanSure RV real-time RT-PCR assay. Methods: We tested 254 respiratory specimens and cultured viral strains using the Luminex xTAG RVP Fast v2 assay (Luminex Molecular Diagnostics, Canada) and Anyplex II RV16 detection kit and compared the results. Specimens showing discordant results between the two assays were tested with a AdvanSure RV real-time RT-PCR assay. Results: Of the 254 respiratory specimens, there was total agreement in the results between the xTAG RVP Fast v2 assay and the other real-time PCR assay in 94.1–100% of the specimens. The agreement levels were relatively low (94.1–97.6%) for specimens of adenovirus, coronavirus NL63, and parainfluenza type 3. In comparison to the other assay, the xTAG RVP Fast v2 assay detected a higher number of parainfluenza type 3 (4 cases) and metapneumovirus (9 cases). Conclusions: The xTAG RVP Fast v2 assay showed comparable capabilities compared with the other assays; it will be useful for identifying respiratory viral infections in patients with respiratory symptoms. Clinicians should be aware of the characteristics of the assays they use, since different assays show different detectability for each virus.
机译：背景：准确，快速地识别出致病病毒对于及时诊断和管理呼吸道感染至关重要。多重分子诊断技术已被广泛用于检测呼吸道病毒。我们将新升级的，基于分子珠的多重，多重，基于分子的呼吸道病毒检测（RVP）分析的结果与Anyplex II RV16检测试剂盒和AdvanSure RV实时RT-PCR分析的结果进行了比较。方法：我们使用Luminex xTAG RVP Fast v2测定法（Luminex Molecular Diagnostics，加拿大）和Anyplex II RV16检测试剂盒测试了254个呼吸道标本和培养的病毒株，并比较了结果。使用AdvanSure RV实时RT-PCR测定法对显示两种测定结果不一致的标本进行了检测。结果：在254个呼吸道标本中，在9TAG–100％的标本中，xTAG RVP Fast v2测定与其他实时PCR测定之间的结果完全一致。对于腺病毒，冠状病毒NL63和3型副流感样本，协议水平相对较低（94.1–97.6％）。与其他分析相比，xTAG RVP Fast v2分析检测到3型副流感的病例较多（4例）和间质肺病毒（9例）。结论：xTAG RVP Fast v2测定法与其他测定法相比具有可比的功能;这对于识别有呼吸道症状的患者的呼吸道病毒感染很有用。临床医生应了解他们使用的检测方法的特征，因为不同的检测方法对每种病毒显示出不同的可检测性。

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