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首页> 外文期刊>Australian Journal of Crop Science >Determination and assesment of the sex chromosomes of male trees of pistachio (Pistacia vera L.) using in vitro culture
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Determination and assesment of the sex chromosomes of male trees of pistachio (Pistacia vera L.) using in vitro culture

机译：阿月浑子（Pistacia vera L.）雄性树性染色体的体外培养测定与评估

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摘要

The chromosome number and karyotype analysis of male trees of Pistacia vera L. was investigated to determine the sex chromosomes. For karyological determination, root tips were obtained from in vitro derived shoots. Afterwards fifty plates of mitosis were used for the karyotyping, the five best metaphase plates were measured for karyotype analysis, karyogram and idiogram. The conclusions obtained from the chromosome studies illustrated that the chromosome complement of P.vera L. has 2n = 30 chromosome pairs with metacentric, submetacentric, subtelocentric and telocentric centromeres. Karyotype analysis revealed that male trees of P.vera L. have heteromorphic sex chromosomes (Xy). The largest pair of chromosomes have different physical features from the other pairs. Neither polyploidy nor satellite was detected on any of these chromosome plates.
机译：研究了黄连木雄性树的染色体数目和核型分析，以确定性染色体。为了进行核学测定，从体外衍生的芽中获得了根尖。之后使用五十个有丝分裂板进行核型分析，并测量五个最佳的中期板进行核型分析，核图和体状图。从染色体研究中得出的结论表明，P.vera L.的染色体补体具有2n = 30个染色体对，它们具有亚中心，亚中心，亚中心和端中心着丝粒。核型分析表明，P.vera L.的雄性树具有异型性染色体（Xy）。最大的一对染色体具有与其他染色体不同的物理特征。在任何这些染色体板上均未检测到多倍体或卫星。

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