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首页> 外文期刊>Australian Journal of Crop Science >Development of a core collection for Sri Lankan cinnamon germplasm based on morphological characterization using an eco-geographic survey
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Development of a core collection for Sri Lankan cinnamon germplasm based on morphological characterization using an eco-geographic survey

机译:利用生态地理调查基于形态特征开发斯里兰卡肉桂种质核心种质

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Cinnamomum verum is the most important spice produced by Sri Lanka. This study was carried out to fill the gap of knowledge on diversity of Sri Lankan cinnamon through morphological characterization. Therefore, an eco-geographic survey in major growing areas followed by the development of a core collection and vegetative propagation of the total collection for ex-situ conservation were carried out. Two hundred and sixty nine cinnamon accessions were collected. The average shoot regeneration frequency of 269 accessions was 47.76% after one month of vegetative propagation. The collection was established at Faculty of Agriculture, University of Ruhuna for further studies. The collection was characterized for 15 quantitative and qualitative characters of leaf, stem and inflorescence among which, a wide variation was detected for leaf characters mainly. Leaf length positively correlated with Leaf width (+0.643) and Petiole length (+0.483). Principal component analysis (PCA) revealed that there were 3 dimensions of PCA explaining 88.85% of total variance. In Hierarchical cluster analysis based on above characters, 269 accessions were grouped into five clusters at rescaled distance of 0.1. A representation of 10% accessions was randomly selected from each cluster to develop a core collection with 33 accessions. The core collection of 33 accessions was clustered into five groups according to the same method at rescaled distance of 0.075. The core collection is a representation of the total collection as clusters of the two dendograms could be overlapped. Morphological characterization and core collection development during this study will be useful for germplasm management, conservation and varietal improvement through breeding programmes.
机译:肉桂是斯里兰卡生产的最重要的香料。进行本研究以通过形态表征来填补关于斯里兰卡肉桂品种多样性的知识空白。因此,在主要产区进行了生态地理调查,随后开发了核心收藏品并进行了无性保护的全部收藏品的无性繁殖。收集了269个肉桂种。营养繁殖一个月后,269份材料的平均芽再生频率为47.76%。该收集品是在Ruhuna大学农业学院建立的,以供进一步研究。该集合的特征在于叶片,茎和花序的15个数量和质量特征,其中主要是叶片特征的变化很大。叶长与叶宽(+0.643)和叶柄长度(+0.483)正相关。主成分分析(PCA)显示,PCA有3个维度,占总方差的88.85%。在基于上述特征的层次聚类分析中,将269个种质按0.1的重新缩放距离分为五个聚类。从每个聚类中随机选择10%的种质代表,以开发33种种质的核心菌种。按照相同的方法,将33个种质的核心收藏品按重新缩放的距离0.075分为五个组。核心集合表示总集合,因为两个树状图的群集可能会重叠。在这项研究过程中的形态学表征和核心种质的发展将有助于通过育种计划进行种质管理,保存和品种改良。

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