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首页> 外文期刊>Australian Journal of Crop Science >Management of leaf spot disease of Stevia rebaudiana Bertoni with antagonistic bacteria
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Management of leaf spot disease of Stevia rebaudiana Bertoni with antagonistic bacteria

机译：用拮抗细菌处理甜叶菊甜叶斑病

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Stevia rebaudiana Bertoni, a natural sweetener contains two main sweetest compounds, stevioside (ST) and rebaudioside A (R-A), tasting about 300 and 450 times sweeter than sucrose, respectively. This commercially important plant also suffers a leaf spot disease caused by the fungus Alternaria alternata in various districts of South Bengal, India. Fluorescent Pseudomonas BRL-1 isolated from the rhizosphere, showed both in vitro and in vivo antagonistic activity against the pathogen. In dual culture bioassay as circular and semicircular patterns, the isolate quantitatively inhibits the growth of the pathogen by about 78% and 69%, respectively. In vitro plate assay detected that the strain produces hydrolytic enzymes, IAA and siderophore. In talc based formulation the strain has adequate shelf life. Experimental data illustrates about 70% survivability of the population after 180 days of storage at 4°C. Foliar application of the talc based formulation of the antagonist in the field condition revealed that the maximum mean disease index reached to 0.653 and 0.663 in 2009 and 2010, which showed around 86% and 87 % reduction in disease severity, respectively when compared to control. Transferring the outcome of this work in the field would benefit the growers by facilitating bioorganic production of this important medicinal plant. Ultimately, the consumers will tend to receive a pesticide free natural sweetener.
机译：天然甜味剂甜叶菊甜叶菊含有两种主要的最甜化合物甜菊糖苷（ST）和莱鲍迪苷A（R-A），分别比蔗糖甜300倍和450倍。这种在商业上很重要的植物还遭受了印度南孟加拉邦各个地区的真菌链格孢引起的叶斑病。从根际分离出的荧光假单胞菌BRL-1表现出对病原体的体外和体内拮抗活性。在双重培养生物测定中，圆形和半圆形模式的分离物分别在数量上抑制了约78％和69％的病原体生长。体外平板测定法检测到该菌株产生水解酶，IAA和铁载体。在基于滑石的制剂中，该菌株具有足够的保存期限。实验数据表明，在4°C下储存180天后，种群的生存率约为70％。在田间条件下以滑石粉为基础的拮抗剂叶面施用显示最大平均疾病指数在2009年和2010年达到0.653和0.663，与对照组相比，疾病严重程度分别降低了约86％和87％。在田间转移这项工作的成果将通过促进这种重要药用植物的生物有机生产而使种植者受益。最终，消费者将倾向于接受不含农药的天然甜味剂。

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