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Predicting hybrid performances from interploidy crosses in Musa species

机译:从Musa种的倍体杂交预测杂种表现

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Models for predicting hybrid performance of progenies from crosses have been developed, but genetic parameters for different Musa populations would have to be estimated from experimental data. Determination of these parameters would be dependent on defining the relationships of progeny to parents in different crosses and identifying parental contributions to offspring. The objective of this work was to estimate genetic relationships based on meiotic mechanisms in parents, and redefine models for predicting hybrid performance for different inter-ploidy crosses in Musa. The study was carried out at the High Rainfall Station of the International Institute of Tropical Agriculture in Onne (4o43’ N, 7o01’E, 10 m above sea level). A breeding population of approximately 2150 individuals was developed from crosses involving tetraploid (4x) and diploid (2x) parental lines. The diameter of pollen grains from parental lines was measured under a light microscope, while the ploidy status of the breeding populations was determined by flow cytometry (FCM) analysis. Tetraploid lines had a higher mean pollen diameter than diploids. However, there were no differences between the diameter of “n” pollen of the 4x and 2x lines. 4x - 2x crosses produced predominantly 3x progenies, whereas 2x – 4x crosses gave mostly 2x progenies, suggesting a pattern of unequal contribution of the parents with respect to 3x progeny from 4x – 2x crosses, but equal parental contribution to 2x progeny from 2x – 4x crosses. The knowledge of parental contributions to progeny was used to redefine models for predicting hybrid performance from interploidy crosses in Musa.
机译:已经开发了预测来自杂交的后代的杂交表现的模型,但是必须从实验数据中估计不同穆萨种群的遗传参数。这些参数的确定将取决于在不同杂交中定义后代与父母的关系并确定父母对后代的贡献。这项工作的目的是基于父母的减数分裂机制来估计遗传关系,并重新定义模型以预测穆萨不同倍体间杂交的杂种表现。该研究是在Onne国际热带农业研究所的高降雨站(北纬4o43′,东7o01′,海拔10 m)上进行的。从涉及四倍体(4x)和二倍体(2x)亲本系的杂交中发展了大约2150个个体的繁殖种群。在光学显微镜下测量来自亲本系的花粉粒的直径,而通过流式细胞术(FCM)分析确定繁殖群体的倍性状态。四倍体品系具有比二倍体更高的平均花粉直径。但是,在4x和2x线的“ n”花粉直径之间没有差异。 4x-2x杂交主要产生3x子代,而2x-4x杂交主要产生2x子代,这表明父母对4x-2x杂交的3x子代的贡献不平等,但父母对2x-4x的2x子代的贡献相等。十字架。父母对后代的贡献的知识被用来重新定义模型,以从穆萨的倍性杂交预测杂种表现。

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