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首页> 外文期刊>Australian Journal of Crop Science >Levels of a dominant Glutathione S-transferase in onion bulbs have a seasonal relationship with physiological inhibitors
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Levels of a dominant Glutathione S-transferase in onion bulbs have a seasonal relationship with physiological inhibitors

机译：洋葱鳞茎中主要谷胱甘肽S-转移酶的水平与生理抑制剂有季节性关系

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摘要

Isoforms of onion bulb GSTs (designated as GSTa, GSTb, as minor, and GSTc, GSTd and GSTe as dominant GSTs) were separated by DEAE-cellulose chromatography, and variation in the activity of dominant GSTs was studied in winter and spring. Among the dominant GSTs, GSTc and GSTd were found to have similar activity levels in both seasons. In case of GSTe, distinct activity levels were observed in winter and spring, where, a decreased activity was found in spring. An expressional relation was observed between GSTe and physiological inhibitor, quercetin-4′-glucoside in onion calli. However, GSTe was not responsive to quercetin-3,4′-diglucoside. The concen- trations of the two inhibitors were also compared in onion bulb tissues collected from winter and spring seasons. Quercetin-4′-glucoside and quercetin-3,4′-diglucoside containing fractions, ethylacetate (EtOAc) and water fraction, were isolated from onion bulb extract, and IC50s of the fractions were measured on GSTc, a sensitive GST to the -1 inhibitors. The IC50s of EtOAc- and water fraction on GSTc (in winter 84 and 283 mg ml fresh tissue, respectively, -1 and in spring 102 and 214 mg ml fresh tissues, respectively) and HPLC profile suggested that the concentration of quercetin-4′-glucoside decreased over time of storage in onion bulb, while the concentration of quercetin- 3,4′-diglucoside increased in spring. Therefore, lower activity of GSTe in onion bulb in spring is due to lower content of quercetin-4′-glucoside in onion bulb tissues, and hence, quercetin-4′-glucoside might have important role in expressing the activity of this GST in onion bulb.
机译：通过DEAE-纤维素色谱分离洋葱鳞茎GST的同工型（分别命名为GSTa，GSTb和GSTc，GSTd和GSTe作为主要GST），并在冬季和春季研究了主要GST活性的变化。在主要的GST中，两个季节的GSTc和GSTd具有相似的活性水平。在GSTe的情况下，在冬季和春季观察到不同的活性水平，其中在春季发现活性降低。在洋葱愈伤组织中观察到GSTe与生理抑制剂槲皮素4'-葡萄糖苷之间的表达关系。然而，GSTe对槲皮素3,4'-二葡萄糖苷没有反应。还比较了从冬季和春季采集的洋葱鳞茎组织中两种抑制剂的浓度。从洋葱鳞茎提取物中分离出含有槲皮素4'-葡萄糖苷和槲皮素-3,4'-二葡萄糖苷的馏分，乙酸乙酯（EtOAc）和水馏分，并在GSTc（对-1敏感的GST）上测量馏分的IC50。抑制剂。乙酸乙酯和水馏分在GSTc上的IC50值（分别在冬季为84和283 mg ml新鲜组织，在-1处以及在春季为102和214 mg ml新鲜组织中的IC50）和HPLC分析表明，槲皮素4'的浓度洋葱鳞茎中葡萄糖苷的含量随贮藏时间的延长而下降，而槲皮素-3,4'-二葡萄糖苷的含量在春季升高。因此，春季洋葱鳞茎中GSTe的活性较低是由于洋葱鳞茎组织中槲皮素-4'-葡萄糖苷的含量较低，因此槲皮素-4'-葡萄糖苷可能在洋葱中表达该GST的活性中起重要作用。灯泡。

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