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首页> 外文期刊>Australian Journal of Crop Science >Anatomical and structural changes in response to application of silicon (Si) in vitro during the acclimatization of banana cv. ‘Grand Naine’
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Anatomical and structural changes in response to application of silicon (Si) in vitro during the acclimatization of banana cv. ‘Grand Naine’

机译：香蕉cv适应过程中响应体外应用硅（Si）的解剖和结构变化。 ‘大纳尼’

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This study aimed at evaluating possible effects of silicon application during in vitro culture of banana plants (cv. ‘Grand Naine’) on structural and anatomical characteristics of leaves of acclimatized plants. Shoots previously established in vitro were inoculated on Murashige and Skoog (MS) medium with addition of 30 g L-1 sucrose and 1 mg L-1 NAA (naphthalene acetic acid) and supplemented with three sources of silicon (sodium silicate, potassium silicate and calcium silicate) at a dosage of 1 g L-1. Medium without any source of silicate was used as a control treatment. The cultures were maintained for 45 days at the rooting stage. After this phase, the plants were transferred to the greenhouse where they were held for 60 days. The experimental design was completely randomized with five replicates (a total of fifteen plants per treatment). The presence of silicate in the culture medium favored increased stomatal density on both sides of the leaves and increased the polar diameter/equatorial diameter ratio on the abaxial side. The thicknesses of mesophyll and chlorophyll content were also enhanced in the presence of silicon, mainly when the source used was the calcium silicate. The use of sodium silicate increased the contents of cellulose, hemicellulose and tannins. The application of silicon during the in vitro culture provides improvements on anatomical and physiological characteristics of leaves of banana plants on acclimatization phase.
机译：这项研究旨在评估在香蕉植物（大纳奈公司）的体外培养过程中施用硅对适应植物叶片结构和解剖特征的可能影响。将先前在体外建立的芽接种在Murashige和Skoog（MS）培养基上，加入30 g L-1蔗糖和1 mg L-1 NAA（萘乙酸），并补充三种硅（硅酸钠，硅酸钾和硅酸钙）的剂量为1 g L-1。没有任何硅酸盐来源的培养基用作对照处理。在生根阶段将培养物维持45天。在此阶段之后，将植物转移到温室中并放置60天。实验设计完全随机化，重复5次（每次处理共15株植物）。培养基中硅酸盐的存在有利于增加叶片两侧的气孔密度，并增加其背面的极径/赤道直径比。在硅的存在下，叶肉和叶绿素含量的厚度也增加了，主要是当使用的来源是硅酸钙时。硅酸钠的使用增加了纤维素，半纤维素和单宁的含量。硅在体外培养过程中的应用改善了香蕉植物在适应阶段叶片的解剖和生理特性。

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