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Cluster analysis to select peanut drought tolerance lines

机译:聚类分析选择花生抗旱品系

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The breeding programs focused on semiarid environments significantly invest on selection of early-mature genotypes with the ability to maintain yield at reasonable levels, when drought sets in. The fastigiata peanut genotypes have broad physiological adaptation to environments prone to drought, while subsp. hypogaea are less tolerant, but are excellent to pod yield. The combination of these intraspecific materials via hybridization often provides robust descendants to breeding works focusing on yield and drought tolerance. In this work we adopted a clustering method to assist in the selection procedures of intraspecific peanut elite lines, based on molecular tools and physiological, biochemical and agronomic traits. Initially, fourteen intraspecific top lines (F7) were used to identify drought tolerant materials based on SSR markers. Thereafter, the selected lines were submitted to 10 days (d) of water stress and analyzed by physiological (stomata conductance, transpiration and photosynthesis) and biochemical (organic solutes and antioxidative enzymes) traits. These data were used to identify lines tolerant to water stress, based on Canonical variable analysis. Additionally, these same lines were used in validation assays during two year experiments, under irrigated and rainfed conditions. We found that all selected top lines showed osmotic adjustment and antioxidant satisfactory front of disturbances caused by water stress. However, based on the clustering analysis the top line L46 was more suitable for semiarid environment, due to agronomic similarity with drought tolerant-BR 1.
机译:着眼于半干旱环境的育种计划大量投资于选择早期成熟的基因型,以便在干旱发生时将产量保持在合理的水平。Fastigiata花生基因型对容易干旱的环境具有广泛的生理适应性,而亚种。鞭毛病的耐受性较差,但荚果产量优异。这些种内材料通过杂交的结合通常为着重于产量和耐旱性的育种工作提供了强大的后代。在这项工作中,我们基于分子工具以及生理,生化和农艺性状,采用了一种聚类方法来协助种内花生优良品系的选择程序。最初,使用14种种内顶线(F7)基于SSR标记鉴定耐旱材料。此后,选择的品系经受了10天(d)的水分胁迫,并通过生理(气孔导度,蒸腾和光合作用)和生化(有机溶质和抗氧化酶)性状进行了分析。这些数据用于根据规范变量分析确定耐水胁迫的品系。此外,在灌溉和雨水条件下的两年实验中,将这些相同的品系用于验证试验。我们发现,所有选定的顶线均显示出渗透调节和抗氧化剂令人满意的抗水分胁迫前沿。但是,基于聚类分析,由于与耐旱BR 1的农艺相似性,顶线L46更适合半干旱环境。

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