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首页> 外文期刊>Australian Journal of Crop Science >Effect of 2,4-D on fruit sugar accumulation and invertase activity in sweet orange cv. Salustiana
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Effect of 2,4-D on fruit sugar accumulation and invertase activity in sweet orange cv. Salustiana

机译：2,4-D对甜橙简历中水果糖积累和转化酶活性的影响。 Salustiana

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Small fruit size is a common problem in Salustiana oranges. Many reports exist on the use of synthetic auxins to enhance citrus fruit size. Few of these studies delve on the effect of auxins on carbohydrate metabolism and its relationship with enzyme activity. Sugar balance is an important trait for fruit quality and depends on the enzymatic metabolism at different tissue levels. The effect of 2,4- Dichlorophenoxyacetic acid (2,4-D) applications on fruit growth, sugar content, and invertase activity was studied on sweet orange fruit on 20-year-old trees of Citrus sinensis (L.) Osbeck cv. Salustiana. Carbohydrates were quantified via High-Performance Liquid Chromatography (HPLC) and the sugars released by the invertase activity with the Somogy–Nelson method. Then, the growth variables of the fruit were characterized (diameter and fresh and dry weight). The results showed that application of 2,4-D (20 mgL-1, 3.6 L per tree) increased fruit size at maturity (5 mm). Application of 2,4-D increased sugar levels in the fruit and influenced the activity of the different invertase isoforms. The increased activity of the acid invertase isoform suggests a direct effect of 2,4-D on the increased fruit sink strength; it is also related to increased consumption of sugars during the initial fruit development stage.
机译：小果实大小是Salustiana橘子的常见问题。关于使用合成植物生长素来增加柑橘类水果的大小，已有许多报道。这些研究很少探讨生长素对碳水化合物代谢的影响及其与酶活性的关系。糖分的平衡是水果品质的重要特征，它取决于不同组织水平的酶促代谢。研究了20岁的柑桔（Osbeck cv。）的甜橙果实上2,4-二氯苯氧基乙酸（2,4-D）对果实生长，糖含量和转化酶活性的影响。 Salustiana。通过高效液相色谱（HPLC）对碳水化合物进行定量，并通过Somogy-Nelson方法通过转化酶活性释放出糖。然后，表征果实的生长变量（直径以及鲜重和干重）。结果表明，施用2,4-D（20 mgL-1，每棵树3.6 L）会增加成熟时的果实大小（5 mm）。 2,4-D的施加增加了水果中的糖含量，并影响了不同转化酶同工型的活性。酸性转化酶同工型的活性增加表明2，4-D对增加的果实贮藏强度有直接作用。它也与水果发育初期糖的消耗增加有关。

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