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首页> 外文期刊>Australian Journal of Crop Science >Evaluation of genetic diversity in acid lime (Citrus aurantifolia Swingle) genotypes using AFLP markers
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Evaluation of genetic diversity in acid lime (Citrus aurantifolia Swingle) genotypes using AFLP markers

机译：使用AFLP标记评估酸性石灰（Citrus aurantifolia Swingle）基因型的遗传多样性

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摘要

Citrus is one of the most important crops of sub-tropical regions worldwide, including acid lime (Citrus aurantifolia). This includes a large group of citrus family. AFLP analysis was done using four primer combinations to monitor genetic relationships between 30 local genotypes and six foreign cultivars. AFLP data analysis revealed the presence of 126 scorable bands which revealed % 69.84 polymorphism. The number of amplified bands for each primer combination was recorded as 26-37 (with an average of 22 bands for each primer combination). The measured polymorphic information content (PIC) had an average of 0.48. The range of similarity was between 0.24 and 0.96. Minimum similarity was found between sweet lime (Citrus limetta) and D8 genotype, while the maximum observed between two genotypes of Minaab region. Samples were not separated using cluster analysis regionally. Instead, samples of Minaab region revealed high level of genetic similarity with one another, in comparison to samples of Manoojan and Darab regions. This is due to limited citrus varieties throughout the region. Using these markers, remarkable genetic diversities between genotypes of acid lime were found. Results showed that Iranian acid lime genotypes have high level of genetic diversity because they are sexually propagated.
机译：柑橘是全世界亚热带地区最重要的作物之一，包括酸石灰（Citrus aurantifolia）。这包括一大批柑橘家庭。 AFLP分析使用四种引物组合进行，以监测30个本地基因型与六个外来品种之间的遗传关系。 AFLP数据分析揭示了126条可评分带的存在，揭示了69.84％的多态性。每个引物组合的扩增条带数目记录为26-37（每个引物组合平均22个条带）。测得的多态信息含量（PIC）的平均值为0.48。相似性范围在0.24至0.96之间。甜石灰（Citrus limetta）与D8基因型之间的最小相似性，而Minaab地区的两种基因型之间的最大相似性。样本未使用区域聚类分析进行分离。相反，与Manoojan和Darab地区的样本相比，Minaab地区的样本彼此之间显示出高度的遗传相似性。这是由于整个地区的柑橘品种有限。使用这些标记，发现了酸性石灰基因型之间显着的遗传多样性。结果表明，伊朗酸性石灰的基因型具有较高的遗传多样性，因为它们是有性繁殖的。

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