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首页> 外文期刊>Australian Journal of Crop Science >Analysis of genetic diversity of gac [Momordica cochinchinensis (Lour.) Spreng] in Southern Vietnam using fruit-morphological and microsatellite markers
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Analysis of genetic diversity of gac [Momordica cochinchinensis (Lour.) Spreng] in Southern Vietnam using fruit-morphological and microsatellite markers

机译：利用果实形态和微卫星标记分析越南南部gac [Momordica cochinchinensis（Lour。）Spreng]的遗传多样性

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Sweet gourd (Momordica cochinchinesis (Lour.) Spreng) (2n = 28) belongs to Cucurbitaceae family and is native to Southeast Asia aswell as Vietnam. It is indigenous throughout Asia and being used as food and for medicinal purpose. The aim of this study was toestimate genetic diversity of gac accessions collected from Southern Vietnam based on 7 fruit-morphological traits and 10 SSRmarkers. The analysis of variance (ANOVA) revealed highly significant differences among 16 gac accessions. The gac accessionsrevealed difference in all fruit-morphological characters. Ten SSR primers showed high level of polymorphic fragments (100%). Atotal of 52 alleles were detected and the number of alleles per locus ranged from 2 to 8 with an average of 5.2 alleles per locus. Thefragment size varied from 150 bp to 610 bp. The polymorphism information contents (PIC) of markers varied from 0.27 to 0.86 withan average of 0.58. Expected heterozygosity (H E ) ranged from 0.11 to 0.86 with a average of 0.40. The mean of observedheterozygosity (H O ) was 0.64 with ranging 0.38–0.94. Nei’s genetic distance coefficient ranged between 0.0 and 1.0 and with themean 0.79. Dendrogram based on UPGMA analysis grouped the 16 gac accessions into three main groups. Combination of fruit-morphological with molecular markers such as microsatellite markers gives a good observation of genetic diversity of gacaccessions.
机译：甜葫芦（Momordica cochinchinesis（Lour。）Spreng）（2n = 28）属于葫芦科，原产于东南亚以及越南。它是整个亚洲的土著居民，被用作食品和药用。这项研究的目的是基于7种水果形态特征和10个SSR标记，估计从越南南部收集的gac品种的遗传多样性。方差分析（ANOVA）显示16 gac入种之间存在极显着差异。 gac种质揭示了所有果实形态特征的差异。十个SSR引物显示出高水平的多态性片段（100％）。共检测到52个等位基因，每个基因座的等位基因数量为2至8，平均每个基因座5.2个等位基因。片段大小从150 bp变化到610 bp。标记的多态性信息含量（PIC）从0.27到0.86，平均为0.58。预期杂合度（H E）为0.11至0.86，平均为0.40。观察到的杂合度（H O）的平均值为0.64，范围为0.38-0.94。 Nei的遗传距离系数介于0.0到1.0之间，主题值为0.79。基于UPGMA分析的树状图将这16个gac种质分为三个主要组。水果形态学与分子标记物（如微卫星标记物）的结合可以很好地观察到糖化合子的遗传多样性。

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