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首页> 外文期刊>Australian Journal of Crop Science >Overexpression of HvNHX2, a vacuolar Na + /H + antiporter gene from barley, improves salt tolerance in Arabidopsis thaliana
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Overexpression of HvNHX2, a vacuolar Na + /H + antiporter gene from barley, improves salt tolerance in Arabidopsis thaliana

机译:HvNHX2(大麦的液泡性Na + / H +反转运蛋白基因)的过表达提高拟南芥的耐盐性

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One possible mechanism to avoid Na+ toxicity in the cytosol is compartmentalization of Na+ ions away from the cytosol. In the present work transgenic Arabidopsis plants overexpressing HvNHX2, a vacuolar Na+/H+ antiporter gene from barley, were generated using Agrobacterium tumefaciens transformation. We show that these plants are able to grow normally in presence of 200 mM NaCl, while wild type Arabidopsis plants showed necrosis. When grown on MS medium containing NaCl, transgenic plants overexpressing HvNHX2 accumulated more Na+ in the shoots, and had longer roots in the early seedling stage. These results show that improved salt tolerance could be achieved by compartmentalization of Na+ in the vacuoles.
机译:避免Na +在细胞质中的毒性的一种可能的机制是将Na +离子与细胞质分开。在本工作中,使用根癌土壤杆菌转化产生了过表达HvNHX2(大麦的液泡Na + / H +反转运蛋白基因)的转基因拟南芥植物。我们表明,这些植物能够在存在200 mM NaCl的情况下正常生长,而野生型拟南芥植物却显示出坏死现象。当在含有NaCl的MS培养基上生长时,过表达HvNHX2的转基因植物在芽中积累更多的Na +,并且在幼苗早期具有更长的根。这些结果表明,通过在液泡中分隔Na +可以提高耐盐性。

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