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首页> 外文期刊>Australian Journal of Crop Science >Micrografting of almond (Amygdalus communis) cultivar ‘Nonpareil’
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Micrografting of almond (Amygdalus communis) cultivar ‘Nonpareil’

机译：杏仁（Nmypardalus communis）品种“ Nonpareil”的微嫁接

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Effects of plant growth regulators (PGRs) were investigated on micropropagation of scions and micrografting of almond (Amygdalus communis) cultivar ‘Nonpareil’. In vitro germinated wild almond seedlings developed from seeds were used as rootstocks. The mature apical or subapical shoot tips of almond cultivar ‘Nonpareil’were used as material for establishment of the microscions cultures. The shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations (0.0, 0.5, 1.0, 1.5, 2.0, 4.0 mg l-1) of N6-benzylaminopurine (BAP). The data showed that the increase in BAP concentration resulted in significant reduction at the shoot regeneration rate. Among all tested groups, the highest regeneration rate was obtained on medium containing 1.0 mg l-1 BAP. The regenerated adventitious shoots from in vitro cultures were cultured on media containing BAP (0.5, 1.0, 1.5 mg l-1) combined with 0.2 and 0.4 mg l-1 indole butyric acid (IBA) separately for development of shoots. The best respond was observed from MS medium supplemented with 1.0 mg l-1 BAP + 0.2 mg l-1 IBA. The effects of BAP and IBA (1.0 mg l–1) were studied on development of micrografted plantlets. Regenerated shoots tips, which were micrografted onto in vitro germinated wild almond seedlings. The results indicated that the most graft rate and new shoots formation were obtained 1.0 mg l–1 BAP. In vitro micrografted plantlets were successfully transferred into commercial plastic pots for acclimatization.
机译：研究了植物生长调节剂（PGR）对接穗的微繁殖和杏仁（Amygdalus communis）品种'Nonpareil'的微移植的影响。从种子发育的体外发芽的野生杏仁幼苗用作砧木。杏仁品种“ Nonpareil”的成熟的顶端或顶端下方的茎尖被用作建立小孢子培养物的材料。将茎尖在补充有不同浓度（0.0、0.5、1.0、1.5、2.0、4.0 mg l-1）N6-苄基氨基嘌呤（BAP）的Murashige和Skoog（MS）培养基上培养。数据表明，BAP浓度的增加导致枝条再生速率显着降低。在所有测试组中，在含有1.0 mg 1-1 BAP的培养基上获得了最高的再生速率。在含有BAP（0.5、1.0、1.5 mg l-1）与0.2和0.4 mg l-1吲哚丁酸（IBA）分别混合的培养基上培养来自体外培养物的再生不定芽，用于发育芽。从补充有1.0 mg l-1 BAP + 0.2 mg l-1 IBA的MS培养基中观察到最佳响应。研究了BAP和IBA（1.0 mg -1）对微移植小植株发育的影响。再生的芽尖端，将其微移植到体外发芽的野生杏仁幼苗上。结果表明，以1.0 mg l–1 BAP获得了最高的嫁接率和新芽的形成。将体外移植的小植株成功地转移到商用塑料盆中进行驯化。

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