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首页> 外文期刊>Australian Journal of Crop Science >Quantitative trait loci for leaf chlorophyll fluorescence traits in wheat
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Quantitative trait loci for leaf chlorophyll fluorescence traits in wheat

机译：小麦叶片叶绿素荧光特征的数量性状基因座

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Chlorophyll fluorescence is closely related to photosystem II (PS II), and chlorophyll fluorescence analysis has become one of the most powerful and widely used techniques that are available to plant physiologists and ecophysiologists. In this article we report the genetic study of leaf chlorophyll fluorescence traits in wheat. 114 wheat recombinant inbred lines (RILs) derived from W-7984×Opata85 were used as samples and QTLs of chlorophyll relative content (C), minimum fluorescence yield of PS II (Fo), maximum fluorescence yield of PS II (Fm), variable chlorophyll fluorescence yield (Fv), maximum quantum yield of PS II (Fv /Fm), and time of achieving maximum fluorescence yield (Tm) were obtained and analyzed. Five QTLs associated with C are detected on 4B, 4D, 6D and 7A, and the relative additive contribution of locus C6D and C7A is 15.60% and 11.04%, respectively; two pairs of interaction loci affecting C are found. Three QTLs are identified for Fo on 1A, 1B and 1D. One QTL for Fv is mapped on 7D, and it is found that five pairs of interaction QTLs influencing Fv. One loci significantly influencing Fm is on chromosome 5A, which acts as a major effect gene and can explain 20.69% of total phenotypic variation for Fm, and closely links with the RFLP marker Xcdo749. There are six QTLs controlling Tm mapped on 2D, 3D, 4A, 4D, 5D and 7D; one pair of interaction QTLs influencing Tm exists. Three pairs of interaction QTLs influence Fv/Fm, showing a general epistatic contribution of 94.62%. The identified QTL markers for chlorophyll fluorescence traits will be useful for understanding the genetics background and marker-assisted selection in wheat photosynthetic traits improving.
机译：叶绿素荧光与光系统II（PS II）密切相关，而叶绿素荧光分析已成为植物生理学家和生态生理学家可用的最强大，应用最广泛的技术之一。在本文中，我们报告了小麦叶片叶绿素荧光特征的遗传研究。使用W-7984×Opata85衍生的114个小麦重组自交系（RIL）作为样品和叶绿素相对含量（C），PS II的最小荧光产量（Fo），PS II的最大荧光产量（Fm）的QTL获得并分析叶绿素荧光产量（Fv），PS II的最大量子产量（Fv / Fm）和达到最大荧光产量（Tm）的时间。在4B，4D，6D和7A上检测到五个与C相关的QTL，C6D和C7A位点的相对加成贡献分别为15.60％和11.04％;发现了两对影响C的相互作用基因座。在1A，1B和1D上为Fo确定了三个QTL。一个Fv的QTL映射在7D上，并且发现影响Fv的五对交互QTL。一个显着影响Fm的位点位于5A染色体上，这是一个主要效应基因，可以解释Fm总表型变异的20.69％，并与RFLP标记Xcdo749密切相关。有六个控制Tm的QTL映射在2D，3D，4A，4D，5D和7D上。存在一对影响Tm的相互作用QTL。三对相互作用的QTL影响Fv / Fm，显示总体上位性贡献为94.62％。确定的叶绿素荧光性状的QTL标记将有助于了解遗传背景和小麦光合性状改良的标记辅助选择。

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