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首页> 外文期刊>Australian Journal of Crop Science >Long-term conservation of potato genetic resources: Methods and status of conservation
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Long-term conservation of potato genetic resources: Methods and status of conservation

机译:马铃薯遗传资源的长期保存:保存方法和现状

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Plant genetic resources (PGRs) play an important role in agriculture, environment protection, cultural property and trade; they need to be conserved. There are two fundamental approaches for the conservation of PGRs: in situ and ex situ. In situ conservation is the conservation of ecosystems and natural habitats and the maintenance and recovery of viable populations of species in their natural surroundings. Ex situ preservation is the storage of seeds or plant materials under artificial conditions to maintain their long term viability and availability for use. Genebanks employ seed storage, field collections of living plants and in vitro storage (tissue culture or cryopreservation) for ex situ preservation of PGR. Storage of orthodox seeds, which are tolerant to low moisture content and low temperatures at appropriate temperature and humidity, is the most convenient ex situ conservation method. Plants that produce recalcitrant seeds or non-viable seeds are conserved in field genebanks as well as in-vitro in slow growth media for short-to-medium term and cryopreservation in liquid nitrogen at -1960C for long-term periods. Cryopreservation is very expensive and needs trained personnel; this could explain why this method is rarely used for conservation of plant genetic resources in most developing countries. Potato tubers are bulky and highly perishable; the crop is generally conserved as clones either in field genebanks (with annual replanting), in-vitro conservation in slow growth media for short-to-medium term and cryopreservation for long term. Field genebanks are expensive to maintain and the crop is exposed to many dangers; hence, cryopreservation is the only feasible method for long term conservation. However, given the high cost of cryopreservation, long-term conservation of potato genetic resources is poorly developed in most resource-poor countries leading to high rates of genetic erosion. This paper looks into the various methods that that can be applied to conserve potato genetic resources and the status of conservation of potatoes in major genebanks and some countries.
机译:植物遗传资源在农业,环境保护,文化财产和贸易中发挥着重要作用;他们需要被保存。保护植物遗传资源有两种基本方法:原地保护和非原地保护。原生境保护是生态系统和自然栖息地的保护以及自然环境中物种的可行种群的维持和恢复。异地保存是指在人工条件下储存种子或植物材料,以保持其长期生存能力和使用可用性。种质库利用种子存储,活植物的田间采集和体外存储(组织培养或冷冻保存)来对PGR进行异地保存。在适当的温度和湿度下耐低水分含量和低温的正统种子是最方便的非原生境保存方法。可以在田间种质库中保存产生顽固种子或不存活种子的植物,并在慢速生长培养基中进行体外短期至中长期保存,并在-1960°C的液氮中长期保存。冷冻保存非常昂贵,需要训练有素的人员。这可以解释为什么在大多数发展中国家中很少使用这种方法来保护植物遗传资源。马铃薯块茎体积大且易腐烂。通常将农作物作为克隆保存在田间种质库中(需每年重新种植),在慢速生长培养基中进行短期至中期的体外保存,并长期进行低温保存。田间种质库的维护成本很高,农作物面临许多危险。因此,冷冻保存是长期保存的唯一可行方法。但是,由于冷冻保存的高昂费用,在大多数资源匮乏的国家中,马铃薯遗传资源的长期保存发展不佳,导致遗传侵蚀的发生率很高。本文探讨了可用于保存马铃薯遗传资源的各种方法以及主要基因库和某些国家中马铃薯的保存状况。

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