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首页> 外文期刊>Australian Journal of Crop Science >Evaluation of biocontrol potential of Pseudomonas and Bacillus spp. against Fusarium wilt of chickpea
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Evaluation of biocontrol potential of Pseudomonas and Bacillus spp. against Fusarium wilt of chickpea

机译：评价假单胞菌和芽孢杆菌属的生物防治潜力。对鹰嘴豆的枯萎病

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In this study, antagonistic effects of 6 isolates of Pseudomonas and 6 isolates of Bacillus genera isolated from rhizosphere of chickpea were evaluated against Fusarium oxysporum f. sp. ciceris as potential biocontrol agents in vitro and in vivo. Fungal inhibition tests were performed using plate assay. Each isolate were tested for the production of protease, siderophore, cyanide hydrogen, indole acetic acid, antifungal volatile and extracellular compound. Twelve isolates were selected according to their high antagonistic efficiency in in vitro which was shown as inhibition zones in the dual-culture assay. According to phenotypic properties, selected isolates were identified as Bacillus subtilis (B1, B6, B28, B40, B99, and B108), Pseudomonas putida (P9 and P10) and P. aeuroginosa (P11, P12, P66 and P112). The ability of bacterial isolates was varied in production of cyanide hydrogen, siderophore, protease and indole acetic acid (IAA). Biocontrol activity and plant growth promotion of bacterial strains were evaluated under greenhouse conditions, in which P. aeuroginosa (P10 and P12), B. subtilis (B1, B6, B28 and B99) and P. aeuroginosa (P12 and B28) provided better control (P ≤ 0.05) than untreated control (15.8-44.8%) in seed treatment and soil-inoculation, respectively. The growth parameters (plant height, fresh and dry weight of plants) were significantly increased by B28, P12 and P112 isolates in both tests compared to the untreated control. Our results indicate that PGPR improve growth parameters in this plant and can help in the biocontrol of pathogen.
机译：在这项研究中，从鹰嘴豆根际分离的6株假单胞菌假单胞菌和6株芽孢杆菌属菌株对尖孢镰刀菌f的拮抗作用进行了评估。 sp。蜡ice作为潜在的体内外生物防治剂。使用平板测定法进行真菌抑制测试。测试每种分离物的蛋白酶，铁载体，氰化物氢，吲哚乙酸，抗真菌挥发物和细胞外化合物的产生。根据其在体外的高拮抗效率选择了十二个分离株，在双重培养试验中显示为抑制区。根据表型特性，选定的分离株被鉴定为枯草芽孢杆菌（B1，B6，B28，B40，B99和B108），恶臭假单胞菌（P9和P10）和aeuroginosa（P11，P12，P66和P112）。细菌分离物的能力因氰化物氢，铁载体，蛋白酶和吲哚乙酸（IAA）的产生而异。在温室条件下评估了细菌菌株的生物防治活性和植物生长促进，其中铜绿假单胞菌（P10和P12），枯草芽孢杆菌（B1，B6，B28和B99）和铜绿假单胞菌（P12和B28）提供了更好的控制（P≤0.05）在种子处理和土壤接种方面分别比未处理的对照组（15.8-44.8％）高。与未经处理的对照相比，B28，P12和P112分离株在两个试验中均显着提高了生长参数（株高，植物的鲜重和干重）。我们的结果表明，PGPR可改善该植物的生长参数，并有助于病原体的生物防治。

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