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首页> 外文期刊>Australian Journal of Crop Science >In vitro selection and characterization of water stress tolerant lines among ethyl methanesulphonate (EMS) induced variants of banana (Musa spp., with AAA genome)
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In vitro selection and characterization of water stress tolerant lines among ethyl methanesulphonate (EMS) induced variants of banana (Musa spp., with AAA genome)

机译:甲烷磺酸乙酯(EMS)诱导的香蕉变种中耐水胁迫品系的体外选择和鉴定(Musa spp。,具有AAA基因组)

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Water stress enforces a serious threat to banana productivity. Therefore, the attempts to develop tolerant lines are of massive worth to increase cop productivity. To develop an efficient in vitro screening of banana for water stress tolerance, twelve EMS – induced variants derived from cultivars; ‘Berangan Intan’ and ‘Berangan’ involving shoot tips were subjected to a stepwise selection procedure contained three levels (10, 20 and 30 g L-1) of PEG induced water stress. Water stress adversely affected fresh weight increase, shoot vigour and multiplication rate. However, L2-5 on the medium fortified with 30 g L -1 PEG, had significantly the greatest fresh weight increase (1.85 ± 0.18 g) while the parental lines (L1-1 and L2-1) exhibited the lowest fresh weight increase (0.29 ± 0.08 and 0.28 ± 0.1 g, respectively). Higher proliferation rate was observed with L2-5 and L1-5 followed by L2-4, L1-4 and L2-6.at each stress level. Proline content was increased noticeably in L2-5 and L1-5 followed by L1-4, L1-6, L2-3, L2-4 and L2-6. A significant decrease in leaf water loss associated with the lessened levels of MDA and H2O2 were recorded with the water stress tolerant lines as compared to susceptible and non – mutated parental lines exposed to PEG. It was concluded that in vitro selection of banana could provide a method for distinguishing the variants for their morphological and physiological responses to water stress whereby L2-5, L1- 5, L2-6, L1-6, L1-4 and L2-3 demonstrated better tolerance against water deficit than the rest and control plants.
机译:水分胁迫严重威胁香蕉的生产力。因此,开发耐性品系的尝试对于提高警察生产力具有巨大的价值。为了开发对香蕉耐水胁迫的有效体外筛选方法,采用了十二个由EMS诱导的变种衍生品种。对涉及芽尖的“ Berangan Intan”和“ Berangan”进行了逐步选择程序,该程序包含三种水平(10、20和30 g L-1)的PEG诱导的水分胁迫。水分胁迫对鲜重增加,嫩芽活力和繁殖率产生不利影响。然而,在强化了30 g L -1 PEG的培养基上,L2-5的鲜重增加最大(1.85±0.18 g),而亲本系(L1-1和L2-1)的鲜重增加最低(分别为0.29±0.08和0.28±0.1 g)。在每个应力水平下,L2-5和L1-5随后是L2-4,L1-4和L2-6观察到更高的增殖率。 L2-5和L1-5中脯氨酸含量显着增加,随后是L1-4,L1-6,L2-3,L2-4和L2-6。与暴露于PEG的易感和非突变亲本品系相比,耐水胁迫品系表明与MDA和H2O2含量降低相关的叶片水分损失显着减少。结论是香蕉的体外选择可以提供一种区分变体的方法,这些变体具有对水分胁迫的形态和生理响应,其中L2-5,L1-5,L2-6,L1-6,L1-4和L2-3表现出比其余和对照植物更好的耐缺水能力。

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