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首页> 外文期刊>Australian Journal of Crop Science >Differential response of three contrasting pea (Pisum arvense, P. sativum and P. fulvum) species to salt stress: assessment of variation in antioxidative defence and miRNA expression
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Differential response of three contrasting pea (Pisum arvense, P. sativum and P. fulvum) species to salt stress: assessment of variation in antioxidative defence and miRNA expression

机译：三种对比豌豆（Pisum arvense，P。sativum和P. fulvum）对盐胁迫的差异响应：评估抗氧化防御和miRNA表达的变化

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Soil salinity is one of the major abiotic stresses leading to crops yield failure. For investigation of salinity response in pea, in vitro cultures of three pea species (Pisum arvense, P. sativum and P. fulvum) were induced. Regenerated shoots of these pea species were grown on a medium with moderate and high NaCl concentrations (75, 120, 150 and 200 mM NaCl) to compare the antioxidative capacity in response to salt stress. Growth reduction was observed in all species and it correlated with the increase of NaCl concentration. After four weeks of treatment, the crude tissue extracts were used for measuring the stress response parameters. Level of lipid peroxidation increased in all three species, but in P. fulvum the effect of NaCl was less pronounced. Total chlorophyll and phenolic content showed differences among the three species, while the DPPH-scavenging activity was the most evident in P. fulvum. The northern blot analysis of the miRNA398 expression showed a similar pattern between P. sativum and P. arvense but different to that of P. fulvum, as in P. fulvum miRNA 398 was only expressed at moderate NaCl concentration, with total inhibition at high concentrations. On the basis of these results it can be concluded that P. fulvum could represent a gene pool for improving P. sativum and P. arvense stress defence capacity.
机译：土壤盐分是导致作物减产的主要非生物胁迫之一。为了研究豌豆中的盐度响应，诱导了三种豌豆（Pisum arvense，P。sativum和P. fulvum）的体外培养。这些豌豆物种的再生芽生长在中等浓度和高浓度NaCl（75、120、150和200 mM NaCl）的培养基上，以比较抗盐胁迫的抗氧化能力。在所有物种中均观察到生长减少，并且其与NaCl浓度的增加相关。治疗四周后，将粗组织提取物用于测量应激反应参数。脂质过氧化的水平在所有三个物种中增加，但在黄腐体育中，NaCl的作用不太明显。这三个物种之间的总叶绿素和酚含量显示出差异，而DPPH清除活性在黄萎病菌中最明显。对miRNA398表达的Northern印迹分析显示，在腐植假茎和拟南芥之间有相似的模式，但与黄腐假单胞菌的模式不同，因为在黄腐假单胞菌中，miRNA 398仅在中等NaCl浓度下表达，在高浓度下总抑制。基于这些结果，可以得出结论，黄花假单胞菌可以代表用于提高栽培假单胞菌和抗狂野假单胞菌胁迫防御能力的基因库。

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