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Infiltrating CD16

机译:浸润CD16

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摘要

Our aim was to characterize glomerular monocytes (Mo) infiltration and to correlate them with peripheral circulating Mo subsets and severity of lupus nephritis (LN). Methods. We evaluated 48 LN biopsy samples from a referral hospital. Recognition of Mo cells was done using microscopic view and immunohistochemistry stain with CD14 and CD16. Based on the number of cells, we classified LN samples as low degree of diffuse infiltration (5 cells) and high degree of diffuse infiltration (a?¥5 cells). Immunophenotyping of peripheral Mo subsets was done using flow cytometry. Results. Mean age was years and the mean SLEDAI was . The most common SLE manifestations were proteinuria (91%) and hypocomplementemia (75%). Severe LN was found in 70% of patients (Class III, 27%; Class IV, 43%). Severe LN patients and patients with higher grade of CD16.
机译:我们的目的是表征肾小球单核细胞(Mo)的浸润,并将其与周围循环的Mo亚群和狼疮性肾炎(LN)的严重程度相关联。方法。我们评估了来自转诊医院的48个LN活检样本。 Mo细胞的识别是通过显微镜观察和CD14和CD16免疫组织化学染色进行的。根据细胞数,我们将LN样本分类为低浸润度(<5个细胞)和高浸润度(a?¥ 5个细胞)。使用流式细胞仪对外周Mo亚群进行免疫分型。结果。平均年龄是岁,平均SLEDAI是。 SLE最常见的表现是蛋白尿(91%)和低补体血症(75%)。在70%的患者中发现了严重的LN(III类,27%; IV类,43%)。严重的LN患者和CD16等级较高的患者。

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