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首页> 外文期刊>Arquivos Brasileiros de Oftalmologia >Detection of Toxoplasma gondii DNA in peripheral blood and aqueous humor of patients with Toxoplasmic active focal necrotizing retinochoroiditis using real-time PCR
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Detection of Toxoplasma gondii DNA in peripheral blood and aqueous humor of patients with Toxoplasmic active focal necrotizing retinochoroiditis using real-time PCR

机译：实时荧光定量PCR检测弓形虫活动性局灶性坏死性脉络膜脉络膜炎患者外周血和房水中的弓形虫DNA

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ABSTRACT Purpose: To evaluate the ability of real-time quantitative PCR (qPCR) for detectingToxoplasma gondii DNA in the peripheral blood and aqueous humor of patients with toxoplasmic active focal necrotizing retinochoroiditis. Methods: Fifty-five patients with infectious uveitis seen from 2009 to 2013 at the Department of Ophthalmology and Visual Sciences of the Federal University of S?o Paulo were enrolled in this study. Forty-three patients had toxoplasmic active focal necrotizing retinochoroiditis, and the remaining 12 had non-toxoplasmic infectious uveitis and served as controls. qPCR analysis forT. gondii DNA was performed on the patients' peripheral blood and aqueous humor samples. Results: The qPCR was positive for T. gondii DNA in 37.21% (16/43) of the aqueous humor samples and 2.33% (1/43) of the peripheral blood samples; further, 16.27% (7/43) of the patients had positive results in both their blood and aqueous humor samples. Conclusion: qPCR was able to detect T. gondii DNA in patients with toxoplasmic active focal necrotizing retinochoroiditis in the blood as well as the aqueous humor and can help with the diagnosis of the disease.
机译：摘要目的：评价实时定量PCR（qPCR）检测弓形虫活动性局灶性坏死性脉络膜脉络膜炎患者外周血和房水中弓形虫DNA的能力。方法：研究对象为2009年至2013年在圣保罗联邦大学眼科和视觉科学系收治的55例感染性葡萄膜炎患者。 43例弓形虫性活动性坏死性视网膜脉络膜炎，其余12例患有非弓形虫性感染性葡萄膜炎并作为对照。 T的qPCR分析。对患者的外周血和房水样本进行了刚地DNA的检测。结果：在房水样品中37.21％（16/43）和外周血样品中2.33％（1/43）的弓形虫DNA的qPCR阳性;此外，16.27％（7/43）的患者的血液和房水样本均呈阳性结果。结论：qPCR能够检测弓形虫活动性局部病灶性坏死性脉络膜脉络膜炎以及房水中的弓形虫DNA，有助于该病的诊断。

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