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首页> 外文期刊>Asian Journal of Plant Sciences >Effect of NaCl on Growth and Development of in vitro Plants of Date Palm ( Phoenix dactylifera L.) ?Khainazi? Cultivar
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Effect of NaCl on Growth and Development of in vitro Plants of Date Palm ( Phoenix dactylifera L.) ?Khainazi? Cultivar

机译：NaCl对枣椰（Phoenix dactylifera L。）“ Khainazi”离体植物生长发育的影响。品种

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Background and Objectives: High salinity of soil and water resources is a global issue which is causing substantial decline of productive lands in many arid and semiarid regions of the world due to salinization. Therefore, the main objective of this study was to determine the effect of NaCl on growth and development of in vitro plants of date palm ( Phoenix dactylifera L.) ?Khainazi? cultivar. Materials and Methods: The study was carried out in the Tissue Culture Laboratory, Date Palm Research Center, King Faisal University, Kingdom of Saudi Arabia. Ten date palm offshoots of cv. Khainazi, about 3-4 years old and weighing 5-7 kg were used in the experiment. The shoot tip and lateral buds were sterilized in 20% v/v clorex solution for 15 min and then sectioned into 1 cm explants for culture initiation. The modified MS media was supplemented with different concentrations of NaCl ranging from 0, 50, 100, 200, 300 and 400 mmol L?1 and incubated at 25±2°C for 16 h in light daily supplied by 65/80 Warm White Weisse 3500 fluorescent tubes. Each treatment was replicated 10 times by following a completely randomized design. Plant observations included dry weight of shoots and roots and length of stem and roots. The concentration of Na, Mg, Ca and K in plant roots and shoots were determined by the Atomic Absorption Spectrophotometer while Cl by a Chloride meter. Experimental data were analyzed by following appropriate statistical procedures. Results: Stem and root length and dry weight were progressively reduced in most of the treatments by the addition of 0, 50, 100, 200, 300 and 400 mmol L?1 to the growth medium. The reduction in shoot, however, was greater than root. The concentration of Na increased significantly both in the shoots and roots. While the concentration of Ca, Mg and K increased with the addition of NaCl up to 100 mmol L?1, then decreased with increasing the NaCl concentration up to 400 mmol L?1 in the growth medium.. Conclusion: Addition of high NaCl concentration to growth medium for the development of date palm off-shoots using tissue culture technology did not show encouraging results. In conclusion, addition of NaCl solution having concentration above 100 mmol L?1 to growth medium proved detrimental to explants to obtain desired date palm off shoots.
机译：背景与目标：高盐分的土壤和水资源是一个全球性问题，由于盐碱化，导致世界上许多干旱和半干旱地区的生产用地大量减少。因此，本研究的主要目的是确定氯化钠对枣椰子（Phoenix dactylifera L。）？Khainazi？体外植物生长和发育的影响。品种材料和方法：该研究在沙特阿拉伯国王费萨尔大学伊达棕榈研究中心的组织培养实验室进行。简历的十个枣椰树分支。实验中使用了大约3-4岁，重5-7公斤的Khainazi。将芽尖和侧芽在20％v / v clorex溶液中灭菌15分钟，然后切成1 cm外植体用于培养起始。改良的MS培养基中添加了不同浓度的NaCl，分别为0、50、100、200、300和400 mmol L 1 ，并在每天提供的光照下于25±2°C孵育16小时65/80暖白色Weisse 3500荧光灯管。按照完全随机的设计，将每种处理重复10次。植物观察包括芽和根的干重以及茎和根的长度。用原子吸收分光光度计测定植物根和茎中的Na，Mg，Ca和K的浓度，用氯离子计测定Cl的浓度。通过遵循适当的统计程序分析实验数据。结果：在大多数处理中，通过向生长培养基中添加0、50、100、200、300和400 mmol L ？1 逐渐减少茎，根长和干重。然而，芽的减少大于根。 Na的浓度在芽和根中均显着增加。随着NaCl的添加，Ca，Mg和K的浓度升高至100 mmol L ？1 ，然后随着NaCl的浓度升高，直至400 mmol L ？1 降低。 >在生长培养基。中。结论：使用组织培养技术向生长培养基中添加高浓度的NaCl来促进枣椰树枝的发育没有显示出令人鼓舞的结果。总之，向生长培养基中添加浓度大于100 mmol L ？1 的NaCl溶液对外植体有害，无法获得所需的枣掌芽。

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