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首页> 外文期刊>IBRO Reports >Expressions of VGLUT1/2 in the inspiratory interneurons and GAD65/67 in the inspiratory Renshaw cells in the neonatal rat upper thoracic spinal cord
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Expressions of VGLUT1/2 in the inspiratory interneurons and GAD65/67 in the inspiratory Renshaw cells in the neonatal rat upper thoracic spinal cord

机译：新生大鼠上胸脊髓吸气中神经元VGLUT1 / 2的表达和吸人Renshaw细胞中GAD65 / 67的表达

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Although the inspiratory spinal interneurons are thought to provide a major fraction of the excitatory synaptic potentials to the inspiratory intercostal motoneurons, this has not been confirmed. To clarify whether some inspiratory spinal interneurons are glutamatergic, we obtained whole-cell recordings from the ventromedial area of the third thoracic segments in an isolated brainstem-spinal cord preparation from neonatal rat, and the recorded cells were filled with Lucifer Yellow for later visualization. We then examined the existence of mRNA of vesicular glutamate transporters 1 and/or 2 (VGLUT1/2) by performing in situ hybridization. To discriminate the interneurons from motoneurons, we electrically stimulated the third thoracic ventral root on the recorded side, and the results verified that the antidromic spike or excitatory postsynaptic potential was not evoked. In cases in which the ventral root stimulation evoked depolarizing postsynaptic potentials, we examined the existence of glutamic acid decarboxylase 65 and/or 67 (GAD65/67) mRNA using a mixed probe to verify whether the cell was truly a Renshaw cell. The long diameter of the recorded interneurons was 22?±?8?μm; the short diameter was 13?±?4?μm. The interneurons' input resistance was 598?±?274?MΩ. The Renshaw cells had similar sizes and input resistance. Six of 11 interneurons expressed VGLUT1/2, and four of five Renshaw cells expressed GAD65/67. Our findings suggest that approximately one-half of the inspiratory interneurons in the ventromedial area of the neonatal rat thoracic spinal cord are glutamatergic, and these interneurons might enhance the inspiratory intercostal motor activity.
机译：尽管认为吸气脊神经中枢能为吸气肋间运动神经元提供大部分的兴奋性突触潜能，但这尚未得到证实。为了阐明某些吸气性脊髓中枢神经元是否是谷氨酸能的，我们从新生大鼠的分离的脑干-脊髓制备了第三胸段腹侧区的全细胞记录，并将记录的细胞充满路西法黄以供以后观察。然后，我们通过进行原位杂交检查了水疱谷氨酸转运蛋白1和/或2（VGLUT1 / 2）的mRNA的存在。为了区分运动神经元和中间神经元，我们用电刺激了记录侧的第三胸腹根，并且结果证实未诱发出抗峰突或兴奋性突触后电位。在腹侧根部刺激引起去极化突触后电位的情况下，我们使用混合探针检查了谷氨酸脱羧酶65和/或67（GAD65 / 67）mRNA的存在，以验证该细胞是否确实是Renshaw细胞。所记录的中间神经元的长直径为22±±8μm;短直径为13±±4μm。中间神经元的输入电阻为598？±？274？MΩ。 Renshaw电池具有相似的尺寸和输入电阻。 11个中间神经元中有6个表达VGLUT1 / 2，5个Renshaw细胞中有4个表达GAD65 / 67。我们的发现表明，新生大鼠胸脊髓腹侧区域中约一半的吸气中枢神经是谷氨酸能的，这些中神经可能增强吸气性肋间运动。

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