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首页> 外文期刊>IBRO Reports >Detection of local and remote cellular damage caused by spinal cord and peripheral nerve injury using a heat shock signaling reporter system
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Detection of local and remote cellular damage caused by spinal cord and peripheral nerve injury using a heat shock signaling reporter system

机译：使用热激信号报告系统检测脊髓和周围神经损伤引起的局部和远端细胞损伤

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摘要

Spinal cord and peripheral nerve injury results in extensive damage to the locally injured cells as well as distant cells that are functionally connected to them. Both primary and secondary damage can cause a broad range of clinical abnormalities, including neuropathic pain and cognitive and memory dysfunction. However, the mechanisms underlying these abnormalities remain unclear, awaiting new methods to identify affected cells to enable examination of their molecular, cellular and physiological characteristics. Here, we report that both primary and secondary damage to cells in mouse models of spinal cord and peripheral nerve injury can be detectedin vivousing a novel fluorescent reporter system based on the immediate stress responseviaactivation of Heat Shock Factor 1. We also provide evidence for altered electrophysiological properties of reporter-positive secondarily-injured neurons. The comprehensive identification of injured, but surviving cells located both close and at distant locations from the injury sitein vivowill provide a way to study their pathophysiology and possibly prevention of their further deterioration.
机译：脊髓和周围神经损伤导致对局部损伤的细胞以及与其功能连接的远距离细胞的广泛损害。原发性和继发性损伤均可引起广泛的临床异常，包括神经性疼痛以及认知和记忆功能障碍。但是，这些异常的潜在机制尚不清楚，正在等待新的方法来鉴定受影响的细胞，以便对其分子，细胞和生理特征进行检查。在这里，我们报道可以使用一种新型的荧光报告系统，通过激活热休克因子1的即时应激反应，在体内检测到小鼠脊髓模型和周围神经损伤中的原发性和继发性损伤。我们还提供了改变电生理的证据阳性继发性损伤神经元的特性体内损伤位置附近和远离位置的受损但存活细胞的全面鉴定将为研究其病理生理学和可能防止其进一步恶化提供一种途径。

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