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首页> 外文期刊>Indian Journal of Medical Microbiology >Emergence of dengue virus 4 as the predominant serotype during the outbreak of 2017 in South India
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Emergence of dengue virus 4 as the predominant serotype during the outbreak of 2017 in South India

机译：2017年印度南部爆发登革热病毒4作为主要血清型

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Context: Dengue virus (DENV) causes acute febrile illness in tropical and subtropical countries. In India there is a steady increase in incidence since 1950s which could be attributed to emergence of new serotype or lineage ade shifts in circulating DENV. Aims: We aimed to perform molecular characterisation and phylogenetic analysis on samples from the recent outbreak (August–October 2017). Settings and Design: Retrospective epidemiological analysis of dengue outbreak. Subjects and Methods: Samples positive for non-steroidal 1 antigen by enzyme-linked immunosorbent assay (n = 147) were included. The study was approved by our institute ethics committee (JIP/IEC/2018/496). Five hundred and eleven base pair of capsid and pre-membrane encoding genes (CprM) region was amplified using Lanciotti primers, followed by second round of polymerase chain reaction using serotype specific primers. Samples which were positive by second round (n = 68) were sequenced and genotyped using Basic Local Alignment Search Tool analysis and phylogenetic tree was constructed by MEGA7 software. Results: Phylogenetic analysis of CprM sequences identified all 4 serotypes in circulation during this outbreak. We observed both single (n = 50) and concurrent infections (n = 18), with DENV4 as the major contributor (64%). Within Genotype I of DENV4 we observed a distinct new clade (Clade E) which was 2.6% ± 0.9%–5.5% ± 1.1% divergent from the other clades. Among the concurrent infection, DENV 4 and DENV 2 combination was observed to form the majority (77.8%). Conclusions: Overall this study documents the emergence of DENV4 as the major serotype in circulation, replacing DENV1, 2 and 3 which had been previously reported from Tamil Nadu and Puducherry. This substantiates the need for continuous monitoring in endemic countries like India, where such data may impact the formulation of vaccine policy for dengue.
机译：背景：登革热病毒（DENV）在热带和亚热带国家引起急性发热性疾病。自1950年代以来，印度的发病率稳步上升，这可能归因于循环DENV中出现了新的血清型或血统。目的：我们旨在对最近爆发的样本（2017年8月至2017年10月）进行分子表征和系统发育分析。设置和设计：登革热暴发的回顾性流行病学分析。受试者和方法：包括通过酶联免疫吸附法（n = 147）对非甾体1抗原呈阳性的样品。该研究得到我们研究所伦理委员会的批准（JIP / IEC / 2018/496）。使用Lanciotti引物扩增了511个衣壳和膜前编码基因（CprM）区域的碱基对，然后使用血清型特异性引物进行了第二轮聚合酶链反应。对第二轮阳性的样本（n = 68）进行测序，并使用基本局部比对搜索工具进行基因分型，并通过MEGA7软件构建系统进化树。结果：CprM序列的系统发育分析确定了这次暴发中循环中的所有4种血清型。我们观察到单次感染（n = 50）和并发感染（n = 18），其中DENV4是主要因素（64％）。在DENV4的基因型I中，我们观察到一个独特的新进化枝（进化枝E），与其他进化枝的差异为2.6％±0.9％–5.5％±1.1％。在并发感染中，观察到DENV 4和DENV 2的组合占大多数（77.8％）。结论：总体而言，该研究记录了DENV4作为循环中的主要血清型的出现，取代了先前从泰米尔纳德邦和Puducherry报道的DENV1、2和3。这证实了在像印度这样的流行国家进行连续监测的必要性，在这些国家，此类数据可能会影响登革热疫苗政策的制定。

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