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首页> 外文期刊>Indian Journal of Virology >Molecular Characterization of Tomato leaf curl Palampur virus and Pepper leaf curl betasatellite Naturally Infecting Pumpkin (Cucurbita moschata) in India
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Molecular Characterization of Tomato leaf curl Palampur virus and Pepper leaf curl betasatellite Naturally Infecting Pumpkin (Cucurbita moschata) in India

机译：印度自然感染南瓜（Cucurbita moschata）的番茄叶卷曲帕兰普病毒和胡椒叶卷曲β卫星的分子表征

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Pumpkin cultivation in India is affected by severe incidence of a yellow vein mosaic disease. Tomato leaf curl New Delhi virus and Squash leaf curl China virus are known to be associated with this disease in India. We were able to identify a third begomovirus—Tomato leaf curl Palampur virus (ToLCPMV), from pumpkin showing typical symptoms of the disease at Varanasi based on the sequence of complete DNA-A genome of the virus. The complete DNA-A sequence of the virus shared more than 99% sequence identity with other ToLCPMV isolates available in the GenBank and clustered with them in the phylogenetic analysis. This betasatellite amplified from the same infected sample has been identified as Pepper leaf curl betasatellite (PepLCB) which also infects chilli in India. There was 92% sequence identity between the two isolates. This is the first report of natural infection of ToLCPMV on pumpkin and association of PepLCB with yellow vein mosaic disease of pumpkin in India.
机译：印度的南瓜种植受到黄色静脉花叶病严重发病的影响。在印度，番茄叶片卷曲的新德里病毒和南瓜叶片卷曲的中国病毒与这种疾病有关。我们能够根据该病毒的完整DNA-A基因组序列，从显示出该病典型症状的南瓜中鉴定出第三种贝莫病毒-番茄叶片卷曲帕兰普病毒（ToLCPMV）。该病毒的完整DNA-A序列与GenBank中提供的其他ToLCPMV分离株具有超过99％的序列同一性，并在系统发育分析中与它们聚集在一起。从同一感染样本中扩增出的这种β卫星已被鉴定为Pepper Leaf curl betasatellite（PepLCB），它也感染了印度的辣椒。两种分离物之间的序列同一性为92％。这是印度ToLCPMV对南瓜的自然感染以及PepLCB与南瓜的黄脉花叶病的关联的首次报道。

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