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Yeast Biofilm as a Bridge Between Medical and Environmental Microbiology Across Different Detection Techniques

机译:酵母生物膜作为医学和环境微生物学之间跨不同检测技术的桥梁

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Medical and environmental microbiology have two distinct, although very short, histories stemming, the first from the pioneering works of Sommelweiss, Pasteur, Lister and Koch, the second mainly from the studies of Bejerink and Winogradsky. These two branches of microbiology evolved and specialized separately producing distinct communities and evolving rather different approaches and techniques. The evidence accumulated in recent decades indicate that indeed most of the medically relevant microorganisms have a short circulation within the nosocomial environment and a larger one involving the external, i.e. non-nosocomial, and the hospital environments. This evidence suggests that the differences between approaches should yield to a convergent approach aimed at solving the increasing problem represented by infectious diseases for the increasingly less resistant human communities. Microbial biofilm is one of the major systems used by these microbes to resist the harsh conditions of the natural and anthropic environment, and the even worse ones related to medical settings. This paper presents a brief outline of the converging interest of both environmental and medical microbiology toward a better understanding of microbial biofilm and of the various innovative techniques that can be employed to characterize, in a timely and quantitative manner, these complex structures. Among these, micro-Raman along with micro-Brillouin offer high hopes of describing biofilms both at the subcellular and supercellular level, with the possibility of characterizing the various landscapes of the different biofilms. The possibility of adding a taxonomic identification of the cells comprising the biofilm is a complex aspect presenting several technical issues that will require further studies in the years to come.
机译:医学和环境微生物学有两个截然不同的历史,尽管历史很短,第一个历史来自索梅尔维斯,巴斯德,李斯特和科赫的开创性著作,第二个主要来自Bejerink和Winogradsky的研究。微生物学的这两个分支分别发展和专门化,产生了独特的群落,并且进化出截然不同的方法和技术。近几十年来积累的证据表明,实际上,与医学有关的大多数微生物在医院环境中的循环很短,而在外部环境(即非医院环境)和医院环境中的微生物更大。该证据表明,方法之间的差异应产生一种趋同的方法,目的是为抵抗力越来越弱的人类社区解决以传染病为代表的日益严重的问题。微生物生物膜是这些微生物用来抵抗自然和人类环境的恶劣条件以及与医疗环境有关的更恶劣条件的主要系统之一。本文简要概述了环境和医学微生物学的共同兴趣,以期更好地了解微生物生物膜以及可用于及时定量地表征这些复杂结构的各种创新技术。在这些之中,微拉曼与微布里渊一起提供了在亚细胞和超细胞水平上描述生物膜的高希望,并具有表征不同生物膜的各种特征的可能性。增加对构成生物膜的细胞进行生物分类鉴定的可能性是一个复杂的方面,提出了若干技术问题,需要在未来几年中进行进一步研究。

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