...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Integrative Medicine International >Influence of Xingnaojing Injection on the Expression of c-fos and c-jun Proteins in Brains of Rats in a Kindling Model of Epilepsy Chronically Induced by Pentetrazol
【24h】

Influence of Xingnaojing Injection on the Expression of c-fos and c-jun Proteins in Brains of Rats in a Kindling Model of Epilepsy Chronically Induced by Pentetrazol

机译：戊四唑慢性诱发癫痫发作模型中醒脑静注射液对大鼠脑c-fos和c-jun蛋白表达的影响

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objective: To investigate the molecular biological mechanism of the antiepileptic effect of Xingnaojing (XNJ) injection on a rat kindling model of epilepsy (KME). Methods: Fifty healthy 6-week-old male Sprague-Dawley rats were divided into the following five groups: blank control (BC) group, model (M) group, XNJ injection (XI) group, phenobarbital injection (PI) group and XNJ combined with phenobarbital (XP) group. There were 10 rats in each group. The intervention drugs were administered 30 min before the model-building drugs once a day for 5 weeks. The model-building drug pentetrazol was given to each group as an intraperitoneal injection 30 min after the use of the intervention drug once a day for 4 weeks for KME establishment (except for the BC group). The BC group was given physiological saline instead. All drugs were injected intraperitoneally. The behaviors of each group of rats were observed after the use of the model-building drugs for 1 h every day. The last kindling test was carried out at the end of week 5. Then, c-fos and c-jun protein expressions in the rat brains of each group were observed and analyzed by immunohistochemistry at the end of the experiment. Results: There was a large number of cells positive for the c-fos and c-jun proteins in the rat brains of the M group. Compared with the M group, the expression level of the c-fos and c-jun proteins was lower in the rat brains of the XI and PI groups (p < 0.01). There was no statistical difference between the XI and PI groups (p > 0.05). The number of positive cells in the rat brains of the XP group was even smaller than that of the XI or PI groups. Conclusion: The antiepileptic effect of the XNJ injection on the rat KME is probably related to its interruption function on the expression of the c-fos and c-jun proteins in rat brains.
机译：目的：探讨醒脑静（XNJ）注射液对大鼠癫痫（KME）点燃模型抗癫痫作用的分子生物学机制。方法：将五十只健康的6周龄雄性Sprague-Dawley大鼠分为以下五个组：空白对照组（BC），模型（M）组，XNJ注射液（XI）组，苯巴比妥注射液（PI）组和XNJ联合苯巴比妥（XP）组。每组有10只大鼠。干预药物在模型药物之前30分钟给药，每天一次，持续5周。建立模型药物的戊四唑在使用干预药物后30分钟每天一次进行腹膜内注射，持续4周用于建立KME（BC组除外）。 BC组改为服用生理盐水。所有药物腹膜内注射。每天使用模型药物1小时后观察每组大鼠的行为。在第5周末进行最后的点燃试验。然后，在实验结束时观察各组大鼠脑中的c-fos和c-jun蛋白表达，并通过免疫组织化学分析。结果：M组大鼠脑中大量的c-fos和c-jun蛋白阳性细胞。与M组相比，XI组和PI组的大鼠脑中c-fos和c-jun蛋白的表达水平较低（p <0.01）。 XI组和PI组之间无统计学差异（p> 0.05）。 XP组大鼠脑中阳性细胞的数量甚至少于XI组或PI组。结论：XNJ注射液对大鼠KME的抗癫痫作用可能与其对大鼠脑c-fos和c-jun蛋白表达的干扰作用有关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号