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Automated bedside flow cytometer for mHLA-DR expression measurement: a comparison study with reference protocol

机译：自动化床旁流式细胞仪用于mHLA-DR表达测量：与参考方案的比较研究

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BackgroundIn various ICU conditions, measurement of diminished expression of human leukocyte antigen-DR on circulating monocytes (mHLA-DR) by flow cytometry appears to be a reliable marker of acquired immunosuppression. Low mHLA-DR is associated with an increased risk of nosocomial infections and mortality. Nevertheless, its use remains somewhat limited and has not been adopted in common medical practice. The main drawback of mHLA-DR measurement is likely related to the use of flow cytometry that is not accessible everywhere on a 24/7 basis. Recently, the Accellix system, a fully automated table top cytometer, was developed for use at bedside or emergency labs. MethodsThe objective was to assess the performance of the Accellix (beta site evaluation including repeatability and method comparison with reference protocol) for the measurement of mHLA-DR expression. ResultsAccellix repeatability at low and high expression levels of mHLA-DR was r 2) ranged from 0.71 to 0.97 ( p ConclusionsThis fully automated table top cytometer appears to be a suitable tool for ICU patient monitoring and on-going clinical trials as there is no sample preparation and no need for specific skills in flow cytometry. Upon validation in a larger cohort study to reinforce reliability, Accellix could represent a major step to make flow cytometry accessible to clinicians by placing the instrument inside intensive care units or emergency laboratories.
机译：背景技术在各种ICU条件下，通过流式细胞仪测量循环单核细胞（mHLA-DR）上人类白细胞抗原-DR表达的降低似乎是获得性免疫抑制的可靠标志。低mHLA-DR与医院感染和死亡的风险增加有关。然而，它的使用仍然受到一定限制，并且在普通医学实践中尚未被采用。 mHLA-DR测量的主要缺点可能与流式细胞术的使用有关，这种流式细胞术并非全天候24/7可用。最近，开发了一种全自动台式细胞仪Accellix系统，可在床头或急诊室使用。方法目的是评估用于测量mHLA-DR表达的Accellix的性能（β部位评估，包括可重复性和与参考方案的方法比较）。结果Accellix在mHLA-DR低表达和高表达水平下的重复性为r 2 ），范围为0.71至0.97（结论结论：这种全自动台式细胞仪似乎是ICU患者监测和持续治疗的合适工具由于无需样品制备，也不需要流式细胞术的特殊技能进行临床试验，在一项较大的队列研究中通过验证以增强可靠性后，通过将仪器放置在重症监护室中，Accellix可能代表了使流式细胞术可供临床医生使用的重要步骤或急诊室。

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