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首页> 外文期刊>International Journal of Advanced Biotechnology and Research >Construction of RNAi Constructs for Soybean to Acquire Resistance against Mungbean Yellow Mosaic India Virus
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Construction of RNAi Constructs for Soybean to Acquire Resistance against Mungbean Yellow Mosaic India Virus

机译：大豆获得对绿豆黄花叶印度病毒抗性的RNAi构建体的构建

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As soybean belongs to legume family, it acts as a predominant source for vegetable protein to human diet.Moreover consumption of soybean improved the health of an individual and provides protection against variouschronic diseases which had been already demonstrated by various studies. Hence, improving the quality andproductivity of soybean has always been an imperative goal for scientists. Still, every year substantial losses insoybean production occur due to pathogen attack particularly, mungbean yellow mosaic India virus. Yellowmosaic disease became a major threat by affecting the yield loss of around $ 300 million dollars in variouslegume crops and in soybean, the yield loss has been estimated about 196.1 million tons. RNAi technologyopens the possibility to raise considerable resistance against viral diseases in major crops. In the present study,we have constructed hairpin loop RNAi vectors which targeting Rep and IR gene of mungbean yellow mosaicIndia virus. The targeted genes were cloned in sense and antisense orientation initially in cloning vectorpHANNIBAL subsequently subcloned in plant transformation binary vector pCAMBIA1301 under the controlof 35S promoter and nos terminator. For plant transformation studies, the constructs were mobilized in toAgrobacteriun tumafaciens strain EHA105 which would be used to raise transgenic soybean plants havingresistant against MYMIV.
机译：由于大豆属于豆科植物，它是人类饮食中植物蛋白的主要来源。此外，食用大豆改善了个体的健康并提供了针对各种慢性疾病的保护，这已被各种研究证明。因此，提高大豆的质量和生产率一直是科学家的当务之急。尽管如此，由于病原体的侵袭，尤其是绿豆黄花叶印度病毒，每年大豆的生产都会大量减少。黄花菜病已成为主要威胁，影响了各种豆类作物和大豆的单产损失约3亿美元，估计单产损失约1.961亿吨。 RNAi技术为提高主要农作物对病毒性疾病的抵抗力提供​​了可能性。在本研究中，我们构建了针对绿豆黄花叶印度病毒的Rep和IR基因的发夹环RNAi载体。首先在克隆载体pHANNIBAL中以有义和反义方向克隆了靶基因，随后在35S启动子和nos终止子的控制下将其亚克隆到植物转化二元载体pCAMBIA1301中。为了进行植物转化研究，将构建体转移到根癌农杆菌菌株EHA105中，该菌株将用于培育对MYMIV具有抗性的转基因大豆植物。

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