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首页> 外文期刊>International Journal Bioautomation >Optical Biosensor with Multienzyme System Immobilized onto Hybrid Membrane for Pesticides Determination
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Optical Biosensor with Multienzyme System Immobilized onto Hybrid Membrane for Pesticides Determination

机译：固定在杂交膜上的多酶光学生物传感器用于农药的测定

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摘要

A construction of optical biosensor based on simultaneous immobilization of acetylcholinesterase and choline oxidase enzymes for the detection of pesticides residues is described. Different kinds of novel SiO2 hybrid membranes were synthesized to be suitable for optical biosensors using sol-gel techniques. The bioactive component of the sensor consists of a multi-enzyme system including acetylcholinesterase and choline oxidase covalently immobilized on new hybrid membranes. The sensor exhibited a linear response to acetylcholine in a concentration range of 2.5 - 30 mM. Inhibition plots obtained from testing carbamate (carbofuran) pesticides exhibited concentration dependent behaviour and showed linear profiles in concentration ranges between 5x10-8 - 5x10-7 M for carbofuran. The factors affecting the constructed optical biosensors were investigated
机译：描述了基于同时固定乙酰胆碱酯酶和胆碱氧化酶的光学生物传感器的结构，用于检测农药残留。利用溶胶-凝胶技术合成了不同种类的新型SiO2杂化膜，适用于光学生物传感器。传感器的生物活性成分由多酶系统组成，包括共价固定在新杂化膜上的乙酰胆碱酯酶和胆碱氧化酶。该传感器在2.5-30 mM的浓度范围内对乙酰胆碱表现出线性响应。从测试氨基甲酸酯（碳呋喃）农药获得的抑制图显示出浓度依赖性的行为，并且在浓度范围为5x10-8-5x10-7 M的呋喃呋喃中显示出线性分布。研究了影响构建的光学生物传感器的因素

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