...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Animal and Veterinary Advances >Differentiation between Vaccinal and Iranian Virulent Isolates of Newcastle Disease Virus based on F Region Genotyping by HRM Analysis
【24h】

Differentiation between Vaccinal and Iranian Virulent Isolates of Newcastle Disease Virus based on F Region Genotyping by HRM Analysis

机译：基于HRM分析的F区基因分型鉴别新城疫病毒疫苗和伊朗毒力分离株。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The purpose of this study is investigate Differentiation Between Vaccinal and Iranian Virulent Isolates of Newcastle Disease Virus based on F Region Genotyping by HRM Analysis. Discrimination of circulating virulent strains of Newcastle Disease Virus (NDV) from low pathogenic and vaccine stains is the basis for implementation of strategies to control and eradication of that aims at the eradication of NDV in poultry. At the present study the applicability of Real time RT-PCR followed High-Resolution Melting-Curve Analysis (HRM) was evaluated for differentiation of pathogenic ND viruses from the live vaccinal strains. Five virulent NDV isolates and 6 live vaccines samples of NDV were used to develop and evaluate the tests. Based on the nucleotide sequence of Fusion (F) gene, 3 primer pairs (B, E and H) were designed and utilized for HRM analysis. The melting temperature of vaccinal strains higher than the virulent isolates were obtained using the B and H primer pairs, but it was not the same as E. Based on the analysis of HRM curves in this study, the B and H primer pairs could better differentiate the vaccinal strains from each other and from virulent isolate than E. As it is found in this study, HRM analysis can differentiate virulent NDVs from live vaccines. In comparison to the existing methods for discrimination of different pathotypes of the ND viruses, the developed test takes a shorter time, needs less infrastructure and facilities and costs significantly less than the other traditional methods like intra cerebral pathogenicity index (ICPI).
机译：本研究的目的是通过HRM分析研究基于F区基因分型的新城疫病毒疫苗和伊朗毒力分离株的区分。从低致病性和疫苗色斑中区分出新城疫病毒（NDV）的循环毒性毒株，是实施旨在根除家禽NDV的控制和根除策略的基础。在当前的研究中，实时荧光定量PCR（RT-PCR）和高分辨熔解曲线分析（HRM）的适用性已得到评估，用于区分活疫苗株中的致病性ND病毒。使用5株强毒NDV分离株和6株NDV活疫苗样本进行了开发和评估。根据Fusion（F）基因的核苷酸序列，设计了3对引物（B，E和H），并用于HRM分析。使用B和H引物对获得的疫苗菌株的解链温度高于强毒分离株，但与E不同。根据本研究中的HRM曲线分析，B和H引物对可以更好地区分正如本研究中发现的那样，HRM分析可以将有毒的NDV与活疫苗区分开。与区分ND病毒不同病原体的现有方法相比，与其他传统方法（如脑内致病性指数（ICPI））相比，开发的测试所用的时间更短，所需的基础设施和设施更少，成本也更低。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号