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首页> 外文期刊>International Journal of Applied Biology and Pharmaceutical Technology >COMPARISON OF TOTAL FLAVONOID, PHENOLIC CONTENT AND ANTIOXIDANT CAPACITY IN LEAF AND SEED EXTRACTS FROM WHITE HOLY BASIL (Ocimum sanctum)
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COMPARISON OF TOTAL FLAVONOID, PHENOLIC CONTENT AND ANTIOXIDANT CAPACITY IN LEAF AND SEED EXTRACTS FROM WHITE HOLY BASIL (Ocimum sanctum)

机译：白圣罗勒叶和种子提取物中总黄酮，酚含量和抗氧化剂能力的比较

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Ocimum sanctum, commonly known as the white holy basil herb belonging to Lamiaceae family is one of the oldest and popular medicinal plant rich in various phytonutrients and antioxidants. In this study, the comparative evaluation of flavonoids, phenolic content, and antioxidant capacity was carried out in methanolic extract prepared from O. sanctum leaves and seeds. The TAC, TPC, and the TFC were measured by ammonium molybdate, Folin-Ciocalteau and aluminum chloride method respectively. Antioxidant activity was also determined by using DPPH and FRAP assay. In response to the above assays, TACs of O. sanctum leaf and seed extracts were 25-248 and 0.011-0.109 μg AAE/10 mg of extract respectively. The TFC assay showed that leaf extract of O. sanctum (14- 225 μg QE/10mg extract) had higher flavonoid content than the seed extract (0.009-0.119 μg QE/10 mg extract) and the TPC assay in the leaf extract (4.49-9.31 μg GAE/mg extract) was higher than those present in seed (4.10-9.05 μg GAE/mg extract). In DPPH assay, % inhibition in O. sanctum leaf extract was determined in the range 18-76% while in seed extract it was 6-29% and in FRAP assay, leaf extract displayed reducing power in range 0.48- 5.50 μg FSE /mg extract while in seed extract it was 0.16-5.46 μg FSE /mg extract . It was observed that O. sanctum leaf extract had high total phenolic and flavonoid content in addition to antioxidant capacity as compared to its seed extract.
机译：圣殿（Ocimum sanctum），通常被称为唇形科的白色罗勒属植物，是最古老且流行的药用植物之一，富含多种植物营养素和抗氧化剂。在这项研究中，对从桔梗叶和种子制备的甲醇提取物中的类黄酮，酚含量和抗氧化能力进行了比较评估。 TAC，TPC和TFC分别通过钼酸铵，Folin-Ciocalteau和氯化铝方法进行测量。还通过使用DPPH和FRAP测定来确定抗氧化活性。响应于上述测定，桔梗叶片和种子提取物的TAC分别为25-248和0.011-0.109μgAAE / 10mg提取物。 TFC分析表明，圣殿叶提取物（14-225μgQE / 10mg提取物）的黄酮含量高于种子提取物（0.009-0.119μgQE / 10 mg提取物）和叶提取物中的TPC分析（4.49） -9.31μgGAE / mg提取物）高于种子中存在的那些（4.10-9.05μgGAE / mg提取物）。在DPPH分析中，桔梗叶提取物的抑制百分数确定在18-76％的范围内，而在种子提取物中则为6-29％，在FRAP分析中，叶提取物的还原力在0.48-5.50μgFSE / mg范围内种子提取物中提取物为0.16-5.46μgFSE / mg提取物。观察到，与其种子提取物相比，圣殿叶提取物除具有抗氧化能力外，还具有较高的总酚和类黄酮含量。

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