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首页> 外文期刊>International Journal of Basic and Applied Sciences >Intra-specific genetic divergence in rapeseed (BRASSICA NAPUS L.) genotypes estimated through SDS-PAGE of total seed proteins
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Intra-specific genetic divergence in rapeseed (BRASSICA NAPUS L.) genotypes estimated through SDS-PAGE of total seed proteins

机译：通过总种子蛋白的SDS-PAGE估算油菜（BRASSICA NAPUS L.）基因型的种内遗传差异

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Through biochemical technique of SDS-PAGE, 136 genotypes of rapeseed (Brassica napus L.) were characterized based on total seed storage proteins. The germplasm used in the study consisted of 135 accessions and one check cultivar obtained from Gene-bank of Plant Genetic Resources Institute (PGRI), NARC, and Islamabad, Pakistan. During this study 12.25% polyacrylamide gels were used and a total of 21 protein sub-units were observed among the genotypes. Out of these 21 bands, 16 (76.19%) were polymorphic and the rest of 5 (23.81%) were monomorphic. The 21 protein sub-units were found within the range of molecular weights from 6 to 180 kDa. The similarity coefficient among these genotypes ranged from 0.83 to 0.98. The genotypes studied were divided into five major clusters through constructing the dendrogram on the basis of dissimilarity matrix using UPGMA (unweighted pair group method with arithmetic averages). Overall a low level of genetic divergence was found. So, it is suggested that to find out high level of genetic diversity among these genotypes 2-D gel electrophoresis along with other modern techniques should be practiced in future because SDS-PAGE technique alone is insufficient to fully explore the genetic diversity present in these genotypes. Keywords : SDS-PAGE; Genotypes; Brassica napus L.; Polymorphic; Monomorphic; Electrophoresis; UPGMA.
机译：通过SDS-PAGE的生化技术，基于总种子贮藏蛋白鉴定了136个基因型油菜（Brassica napus L.）。该研究中使用的种质包括135个种质和1个从NARC植物遗传资源研究所（PGRI）和巴基斯坦伊斯兰堡的基因库获得的检查品种。在这项研究中，使用了12.25％的聚丙烯酰胺凝胶，并且在基因型中共观察到21个蛋白质亚基。在这21个条带中，有16条（76.19％）是多态的，其余5条（23.81％）是单态的。发现21个蛋白质亚基的分子量在6至180kDa的范围内。这些基因型之间的相似系数在0.83至0.98之间。通过使用UPGMA（具有算术平均值的非加权对群方法）在不相似矩阵的基础上构建树状图，将研究的基因型分为五个主要簇。总体而言，发现了较低的遗传差异。因此，建议将来在这些基因型中寻找高水平的遗传多样性2-D凝胶电泳以及其他现代技术应在将来实践，因为仅靠SDS-PAGE技术不足以充分探索这些基因型中存在的遗传多样性。关键字：SDS-PAGE;基因型甘蓝型油菜;多态单态电泳; UPGMA。

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