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首页> 外文期刊>International Journal of Biochemistry Research & Review >Effect of Natural Products on Some Glycosidasesand Their Expected Hypoglycemic Potential
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Effect of Natural Products on Some Glycosidasesand Their Expected Hypoglycemic Potential

机译：天然产物对某些糖苷酶的影响及其预期的降血糖潜力

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Aims: It is to screen many natural product extracts for their in vitro and in vivo effects on the activities of hepatic α-amylase and α-glucosidase to validate their biological importance.Study Design: Different groups of non-diabetic and diabetic rats were treated by different plants for the in vivo study of glycosidases. In vitro effect of the plants on the tested enzymes was studied in presence and absence of their aqueous extract.Place and Duration of Study: Department of Biological & Environmental Sciences, Faculty of Science, Beirut Arab University, Beirut, Lebanon, between March 2013 and February 2014.Methodology: Enzymes were extracted from the livers of normal rats, also the natural products extracts were prepared for the in vitro studies. α-Amylase and α-glucosidase assays were done in the presence and absence of each plant extract. For the in vivo studies, normal non-diabetic rats were divided into groups, whereas the first group is a control that includes rats fed on normal food diet. The other groups include rats fed on normal food diet mixed with the tested plant leaves (20 mg/g body weight/day) Diabetes was induced in diabetic rats by single intraperitonial injection of streptozotocin. Diabetic rats were divided into groups and treated like the non-diabetic rats. Results: Only Thymus vulgaris and Origanum vulgare extracts showed a significant in vitro dose-dependent inhibition on α-amylase with IC50 values of 0.2±0.01 and 0.37±0.03mg/ml, respectively. However, the in vivo effect was not detected for four weeks treatment for the two enzymes. The in vitro treatment of α-amylase by Thymus vulgaris and Origanum vulgare extracts exhibited a mixed-type inhibition. Moreover, the in vivo inhibition of both extracts on the tested hepatic enzymes was not detected in streptozotocin-induced diabetic rats fed on Thymus vulgaris and Origanum vulgare for four weeks. Blood sugar level was non-significantly decreased with respect to that of non-treated rats.Conclusion: some non anti-diabetic plant extracts possess an in vitro inhibition of glycosidases.
机译：目的：筛选许多天然产物提取物对肝α-淀粉酶和α-葡萄糖苷酶活性的体外和体内作用，以验证其生物学重要性。研究设计：治疗了不同组的非糖尿病和糖尿病大鼠由不同植物用于糖苷酶的体内研究。在有水提取物存在和不存在的情况下研究了植物对受试酶的体外作用研究地点和持续时间：2013年3月至2004年3月在黎巴嫩贝鲁特阿拉伯贝鲁特阿拉伯大学理学院生物与环境科学系2014年2月。方法：从正常大鼠的肝脏中提取酶，并制备天然产物提取物用于体外研究。在每种植物提取物的有无下进行α-淀粉酶和α-葡萄糖苷酶测定。对于体内研究，将正常的非糖尿病大鼠分为几组，而第一组是对照组，其中包括以正常食物饮食喂养的大鼠。其他组包括用正常食物饮食喂养的大鼠与被测植物叶片混合（20 mg / g体重/天），通过单次腹膜内注射链脲佐菌素在糖尿病大鼠中诱发糖尿病。将糖尿病大鼠分为几组，并像非糖尿病大鼠一样进行治疗。结果：只有百里香和牛至提取物对α-淀粉酶具有显着的剂量依赖性体外抑制作用，IC50值分别为0.2±0.01和0.37±0.03mg / ml。但是，对于这两种酶处理四个星期都没有检测到体内作用。百里香和牛至提取物对α-淀粉酶的体外处理表现出混合型抑制作用。此外，在以百里香和牛至饲喂普通百日草4天的链脲佐菌素诱导的糖尿病大鼠中，未检测到两种提取物对测试的肝酶的体内抑制作用。相对于未治疗的大鼠，血糖水平没有明显降低。结论：某些非抗糖尿病植物提取物具有糖苷酶的体外抑制作用。

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