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首页> 外文期刊>International Journal of Biosciences >Isolation and molecular identification of hydrocarbon degrading bacteria from oil-contaminated soil
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Isolation and molecular identification of hydrocarbon degrading bacteria from oil-contaminated soil

机译：油污染土壤中降解烃类细菌的分离与分子鉴定

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The spills of hydrocarbon due to the petrochemical industry are major contaminants in the environment. Bioremediation is an effective, economical and environmentally sound treatment. The purpose of our study was to isolate, screen and identified the hydrocarbon degrading bacteria from oil polluted soil. Three oil contaminated soil were collected from Arzew oil refinery, North-west of Algeria. Sixteen bacterial strains were isolated using mineral salt media supplemented with 1% of crude oil; these isolates were screened for their best degradation abilities. Four selected bacterial strains designated as (P2.3, P2.2, S15.1 and E1.1) were identified on the basis of morphological, biochemical and molecular characterization using 16S rRNA gene sequence analysis. The sequences were compared to the closest relative species in the GenBank database of National Centre for Biotechnology Information. The growths rates of the selected isolates were determined using spectrophotometer at 600nm.Based on the partial 16S rRNA gene sequencing and phylogenetic analysis; the isolates were identified as Pseudomonas aeruginosa P2.3, Achromobacter xylosoxidans P2.2, Staphylococcus haemolyticus S15.1 and Enterococcus faecalis E1.1. Results indicated that the isolates strains had effectively utilize crude oil as sole carbon source. Linear increase in Optical Density (OD) was observed between days 4 and 10. Pseudomonas aeruginosa P2.3 and Achromobacter xylosoxidans P2.2 showed the highest growth in media with crude oil. This study indicates that the contaminated soil samples contain a diverse population of hydrocarbon degrading bacteria and these strains could be used for the bioremediation of oil contaminated soil.
机译：石油化学工业造成的碳氢化合物泄漏是环境中的主要污染物。生物修复是一种有效，经济和对环境无害的治疗方法。我们研究的目的是从油污染土壤中分离，筛选和鉴定可降解碳氢化合物的细菌。从阿尔及利亚西北部的Arzew炼油厂收集了三种受石油污染的土壤。使用添加了1％原油的矿物盐培养基分离了16个细菌菌株。筛选这些分离物的最佳降解能力。使用16S rRNA基因序列分析，在形态，生化和分子表征的基础上，鉴定出四个指定菌株（P2.3，P2.2，S15.1和E1.1）。将序列与国家生物技术信息中心的GenBank数据库中最接近的相对物种进行比较。通过分光光度计在600nm处确定所选菌株的生长速率。基于部分16S rRNA基因测序和系统发育分析;分离株被鉴定为铜绿假单胞菌P2.3，木糖氧化无色杆菌P2.2，溶血性葡萄球菌S15.1和粪肠球菌E1.1。结果表明，分离株已有效利用原油作为唯一碳源。在第4天和第10天之间观察到了光密度（OD）的线性增加。铜绿假单胞菌P2.3和木糖氧化无色杆菌P2.2在含原油的培养基中显示出最高的增长。这项研究表明，被污染的土壤样品中含有各种各样的降解碳氢化合物的细菌，这些菌株可用于油污染土壤的生物修复。

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