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Introduction of FRET Application to Biological Single-molecule Experiments

机译:FRET在生物单分子实验中的应用介绍

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Since the fluorescence resonance energy transfer (FRET) measurement is based on the fluorescence measurement technology, the signal can be obtained from single molecules. The single-molecule FRET (smFRET) measurement has already been extensively used in the field of biology. The combination of the single-molecule sensitivity, the nanometer-scale spatial resolution and the realtimeness gives us the exclusive information on the structure and the dynamics of biomolecules, such as coexisting multiple conformational states or temporal evolution of conformational changes. As an introductory review of the smFRET measurement, this article briefly explains the theory, the basic apparatus, the typical data analysis methods and some examples of experiments applied to biomolecules.
机译:由于荧光共振能量转移(FRET)测量基于荧光测量技术,因此可以从单个分子获得信号。单分子FRET(smFRET)测量已经在生物学领域广泛使用。单分子敏感性,纳米级空间分辨率和实时性的结合为我们提供了有关生物分子的结构和动力学的专有信息,例如共存的多个构象状态或构象变化的时间演化。作为对smFRET测量的介绍性综述,本文简要介绍了理论,基本设备,典型数据分析方法以及一些应用于生物分子的实验示例。

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