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首页> 外文期刊>International Journal of Biomedical Science >Quantitation of 6-, 8- and 10-Gingerols and 6-Shogaol in Human Plasma by High-Performance Liquid Chromatography with Electrochemical Detection
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Quantitation of 6-, 8- and 10-Gingerols and 6-Shogaol in Human Plasma by High-Performance Liquid Chromatography with Electrochemical Detection

机译：高效液相色谱-电化学检测技术定量测定人血浆中的6-，8-和10-姜醇和6-Shogaol

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摘要

Zingiber officinale is one of the most commonly used spices. We developed a method to determine the main pungent ginger constituents, 6-, 8- and 10-gingerols and 6-shogaol in human plasma. Quantitation was achieved using a reversed-phase C18 column using high-performance liquid chromatography with electrochemical detection. The assay was linear from 0.1 to 5.0 μg/mL. The within-day coefficients of variation for the assay at 5.0 μg/mL were = 5% for all analytes. The recovery of all four analytes was > 99% for at 5.0 μg/mL. The lower limit of quantitation was 0.1 μg/mL except for 10-gingerol which was 0.25 μg/mL. Currently, there is no analytical method for detecting pungent ginger constituents in human plasma. This HPLC method allows for the detection of all four of ginger’s pungent constituents simultaneously in a relatively short run time of 25 minutes. This method should be useful for determining plasma levels of 6-, 8-, 10-gingerol and 6-shogaol in phase I clinical trials.
机译：姜姜是最常用的香料之一。我们开发了一种确定人血浆中主要刺激性生姜成分，6-，8-和10-姜醇和6-少醇的方法。使用反相C18色谱柱和高效液相色谱仪进行电化学检测，定量。该测定法在0.1至5.0μg/ mL之间呈线性关系。对于所有分析物，在5.0μg/ mL下测定的日内变异系数= 5％。在5.0μg/ mL下，所有四种分析物的回收率均> 99％。定量下限为0.1μg/ mL，除了10-姜油为0.25μg/ mL。当前，没有用于检测人血浆中刺激性姜成分的分析方法。这种HPLC方法可以在25分钟内的相对较短的时间内同时检测出所有四种姜辛辣成分。该方法在I期临床试验中确定6-，8-，10-姜油酚和6-shogaol的血浆水平应该是有用的。

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