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首页> 外文期刊>International Journal of Biomedical Science >A New Spectrophotometric Method for Determination of Phenylpropanolamine HCl in its Pharmaceutical Formulations via Reaction with 2,3,5,6-tetrachloro-1,4-benzoquinone
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A New Spectrophotometric Method for Determination of Phenylpropanolamine HCl in its Pharmaceutical Formulations via Reaction with 2,3,5,6-tetrachloro-1,4-benzoquinone

机译：与2,3,5,6-四氯-1,4-苯醌反应的分光光度法测定药物中的苯丙醇胺盐酸盐

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摘要

A selective and simple spectrophotometric method has been developed for the determination of phenylpropanolamine HCl (PPA) in its dosage forms. The method was based on the formation of a colored N-vinyl chlorobenzoquinone derivative of PPA through its reaction with 2,3,5,6-tetrachloro-1,4-benzoquinone in presence of acetaldehyde. The colored product exhibits maximum absorbance at 650 nm. Different experimental parameters affecting formation and stability of the product were carefully studied and optimized. The factors affecting the reaction were studied and optimized. The stoichiometry of the reaction was determined, and the reaction pathway was postulated. The absorbance concentration plot was rectilinear over the range of 5-100 μg/mL with Limit of Detection (LOD) and Limit of Quantitation (LOQ) of 0.244 μg/mL and 0.74 μg/mL respectively. The analytical performance of the method was fully validated, and the results were satisfactory. The proposed method was successfully applied to the determination of PPA in its commercial dosage forms including tablets, capsules and syrups with good recoveries. Statistical comparison of the results with those of the comparison method showed good agreement and proved that there was no significant difference in the accuracy and precision between the reference and the proposed methods. The mechanism of the reaction pathway was postulated.
机译：已经开发出一种选择性简便的分光光度法，用于测定剂型中的苯丙醇胺盐酸盐（PPA）。该方法基于PPA与2,3,5,6-四氯-1,4-苯醌在乙醛存在下的反应，形成了有色的PPA N-乙烯基氯苯醌衍生物。有色产品在650 nm处显示最大吸光度。仔细研究和优化了影响产品形成和稳定性的不同实验参数。研究和优化了影响反应的因素。确定反应的化学计量，并假定反应路径。吸光度浓度图在5-100μg/ mL范围内呈线性，检测限（LOD）和定量限（LOQ）分别为0.244μg/ mL和0.74μg/ mL。该方法的分析性能得到了充分验证，结果令人满意。所提出的方法已成功用于商业剂量形式的PPA的测定，包括片剂，胶囊剂和糖浆，回收率高。结果与比较方法的统计比较显示出良好的一致性，并证明参考方法和建议方法之间的准确性和精密度没有显着差异。推测了反应途径的机理。

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