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首页> 外文期刊>International Journal of Biomedical Science >Claudin 1 and Claudin 7 Gene Polymorphisms and Protein Derangement are Unrelated to the Growth Pattern and Tumor Volume of Colon Carcinoma
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Claudin 1 and Claudin 7 Gene Polymorphisms and Protein Derangement are Unrelated to the Growth Pattern and Tumor Volume of Colon Carcinoma

机译：Claudin 1和Claudin 7基因多态性和蛋白质排列与结肠癌的生长方式和肿瘤体积无关

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Tight junctions together with adherens junctions are important for preserving tissue integrity. In tumors the normal tissue structure is lost which results in a disorganization and change of phenotype. In this study we assessed the complexity of the invasive front of colon carcinoma using an objective morphometrical technique based on the estimation of fractal dimension and number of free tumor cell clusters. The complexity of the invasive front was correlated to Claudin 1 and Claudin 7 protein expression as well as genetic polymorphisms of their genes. Thirty-three colon carcinomas were used. Images from the invasive front of the tumors were captured and used to calculate a complexity index of the invasive front. The tight junction proteins Claudin 1 and Claudin 7 were stained immunohistochemically in the tumor and in the surrounding normal mucosa. Screening of their genes was performed using DNA sequencing. A significant aberration of protein expression was seen for both Claudin 1 and Claudin 7 compared to normal mucosa. Both homozygous and heterozygous polymorphisms in exon 2 of claudin 1 were found. In claudin 7 a homozygous polymorphism was seen in exon 4. All individuals with tumors that showed either of these polymorphisms also showed the same polymorphism in the adjacent normal mucosa. A significant correlation was found between polymorphisms in CLDN 7 and tumor differentiation p
机译：紧密连接和粘附连接对于保持组织完整性很重要。在肿瘤中，正常的组织结构丢失，导致表型混乱和变化。在这项研究中，我们基于分形维数和游离肿瘤细胞簇数量的估计，使用客观形态计量学技术评估了结肠癌浸润前线的复杂性。侵入性前沿的复杂性与Claudin 1和Claudin 7蛋白表达及其基因的遗传多态性相关。使用了33个结肠癌。捕获来自肿瘤的侵入性前沿的图像，并将其用于计算侵入性前沿的复杂性指数。紧密连接蛋白Claudin 1和Claudin 7在肿瘤和周围正常粘膜中进行了免疫组织化学染色。使用DNA测序对它们的基因进行筛选。与正常粘膜相比，Claudin 1和Claudin 7的蛋白质表达均出现明显异常。在claudin 1的外显子2中发现了纯合和杂合多态性。在claudin 7中，在第4外显子上观察到了纯合多态性。所有肿瘤患者均显示出这些多态性中的任何一个，在相邻的正常黏膜中也显示出相同的多态性。发现CLDN 7中的多态性与肿瘤分化p之间存在显着相关性

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