• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Biomedical Science >Assessment of Pharmacokinetics and Toxicology of Sadat-Habdan Mesenchymal Stimulating Peptide (SHMSP) in Rats and Goats
【24h】

Assessment of Pharmacokinetics and Toxicology of Sadat-Habdan Mesenchymal Stimulating Peptide (SHMSP) in Rats and Goats

机译：Sadat-Habdan间充质刺激肽（SHMSP）在大鼠和山羊中的药代动力学和毒理学评估

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Sadat-Habdan Mesenchymal Stimulating Peptide (SHMSP) was discovered and patented with USPTO in 2008. Studies have shown that SHMSP works as an angiogenesis factor. This study was done to evaluate pharmacokinetics (PK) in rats and toxicology studies in goats and rats. Methods: In 80 skeletally mature Sprague Dawley rats 5 milligram/kg body weight of SHMSP was injected intramuscularly. Blood samples were collected at 0, 30, 60, 120, 240, 320 minutes and 480 minutes. The plasma calibration curves were prepared at concentrations of 6.25, 3.12, 1.56, 0.78 and 0.39 ng/mL by spiking 190 µL of rat plasma with 10µL of working standard and 200 µL of deionized water. Samples were vortexed for five seconds, centrifuged at 14000 rpm for 30 minutes at 4°C and the supernatant was collected analyzed using High-performance liquid chromatography (HPLC). After injection of 20 µL sample, the peptide was eluted with 15ml linear gradient up to 36% of eluent A. The time between injections was 25 min. and the eluent was monitored at a wavelength of 215 nm. The concentration of peptide present in the rat plasma samples collected at different time intervals were quantified using standard curve method. The goats were injected deep intramuscularly 100 mg/kg-body weight of the SHMSP dissolved in injection solution. In 10 Sprague Dawley rats of ≥250 grams of weight, 20 mg/kg/day SHMSP was injected for 7 consecutive days. All the animals were kept at a close watch. Clinical observation at least once daily and as necessary was undertaken. After 2 weeks animals were euthanized and major organs were harvested and histopathology samples were obtained and processed. Results: There were no deaths is either of the study and control group of animals. The gross observations of the various organs appeared normal and histopathological studies did not show any toxicity in the organs tested.  The plasma concentration-time profile of SHMSP after intramuscular injection, the level of SHMSP in an initial high phase reaching the highest at 30 minutes 2.3184 ng/ml and 60 minutes 1.7447 ng/ml at 60 minutes. The lowest level was at 360 minutes of 0.0879 ng/ml. Conclusions: The dose of SHMSP at 20 times the recommended dose was not toxic and secondly the peak time and level was at 30 minutes to 120 minutes and the plasma half-life of SHMSP was 90 minutes.
机译：背景：Sadat-Habdan间充质刺激肽（SHMSP）被发现并于2008年获得USPTO的专利。研究表明，SHMSP是血管生成因子。进行这项研究是为了评估大鼠的药代动力学（PK）以及山羊和大鼠的毒理学研究。方法：对80只骨骼成熟的Sprague Dawley大鼠进行肌肉注射5毫克/千克体重的SHMSP。在0、30、60、120、240、320分钟和480分钟采集血液样本。通过向190 µL大鼠血浆中加入10 µL工作标准液和200 µL去离子水，制备浓度为6.25、3.12、1.56、0.78和0.39 ng / mL的血浆校准曲线。将样品涡旋五秒钟，在4°C下以14000 rpm离心30分钟，并使用高效液相色谱（HPLC）收集上清液。进样20 µL样品后，用15ml线性梯度洗脱肽，最高洗脱液A为36％。进样之间的时间为25分钟。并在215 nm波长处监测洗脱液。使用标准曲线方法对在不同时间间隔收集的大鼠血浆样品中存在的肽浓度进行定量。给山羊深部肌肉注射100 mg / kg体重的溶解于注射液中的SHMSP。在10只体重≥250克的Sprague Dawley大鼠中，连续7天注射20 mg / kg /天的SHMSP。所有动物都受到密切监视。每天至少在必要时进行一次临床观察。 2周后，对动物实施安乐死并收获主要器官，并获得组织病理学样品并进行处理。结果：研究组和对照组均无死亡。各个器官的总体观察结果看来是正常的，而组织病理学研究并未显示受测器官有任何毒性。肌内注射后SHMSP的血浆浓度-时间曲线，初始高阶段的SHMSP水平在30分钟达到最高，为2.3184 ng / ml，60分钟达到1.7447 ng / ml，在60分钟时达到最高。最低水平是360分钟时的0.0879 ng / ml。结论：SHMSP剂量为推荐剂量的20倍无毒，其峰值时间和水平为30分钟至120分钟，SHMSP的血浆半衰期为90分钟。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号