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首页> 外文期刊>International Journal of Breast Cancer >Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in Lebanon
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Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in Lebanon

机译：免疫组化与芯片基因表达谱在黎巴嫩乳腺癌患者中的雌激素受体，孕激素受体和HER2受体状态之间的一致性

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Introduction. Accurate evaluation of estrogen and progesterone receptors and HER2 is critical when diagnosing invasive breast cancer for optimal treatment. The current evaluation method is via immunohistochemistry (IHC). In this paper, we compared results of ER, PR, and HER2 from microarray gene expression to IHC in 81 fresh breast cancer specimens. Methods. Gene expression profiling was performed using the GeneChip Human Genome U133 Plus 2.0 arrays (Affymetrix Inc). Immunohistochemical staining for estrogen receptor, progesterone receptor, and HER2 status was performed using standard methods at a CAP-accredited pathology laboratory. Concordance rates, agreement measures, and kappa scores were calculated for both methods. Results. For ER, Kappa score was 0.918 (95% CI, 0.77.3–1.000) and concordance rate was 97.5% (95% CI, 91.4%–99.7%). For PR, Kappa score was 0.652 (95% CI, 0.405–0.849) and concordance rate was 86.4% (95% CI, 77%–93%). For HER2, Kappa score was 0.709 (95% CI, 0.428–0.916) and concordance rate was 97.5% (95% CI, 91.4%–99.7%). Conclusion. Our results are in line with the available evidence with the concordance rate being the lowest for the progesterone receptor. In general, microarray gene expression and IHC proved to have high concordance rates. Several factors can increase the discordance rate such as differences in sample processing.
机译：介绍。在诊断浸润性乳腺癌以寻求最佳治疗时，准确评估雌激素和孕激素受体以及HER2至关重要。当前的评估方法是通过免疫组织化学（IHC）。在本文中，我们比较了81例新鲜乳腺癌标本中ER，PR和HER2从微阵列基因表达到IHC的结果。方法。使用GeneChip Human Genome U133 Plus 2.0阵列（Affymetrix Inc）进行基因表达谱分析。使用标准方法在CAP认可的病理实验室进行雌激素受体，孕激素受体和HER2状态的免疫组织化学染色。两种方法均计算出一致性率，一致性测度和kappa分数。结果。对于ER，Kappa得分为0.918（95％CI，0.77.3-1.000），一致性率为97.5％（95％CI，91.4％-99.7％）。对于PR，Kappa得分为0.652（95％CI，0.405-0.849），一致性率为86.4％（95％CI，77％-93％）。对于HER2，Kappa得分为0.709（95％CI，0.428-0.916），一致性率为97.5％（95％CI，91.4％-99.7％）。结论。我们的结果与现有证据一致，孕酮受体的一致率最低。通常，微阵列基因表达和IHC被证明具有很高的一致性。诸如样品处理中的差异等多种因素会增加不一致率。

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