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首页> 外文期刊>International Journal of Clinical and Experimental Pathology >Caveolin-1 regulates cell apoptosis and invasion ability in paclitaxel-induced multidrug-resistant A549 lung cancer cells
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Caveolin-1 regulates cell apoptosis and invasion ability in paclitaxel-induced multidrug-resistant A549 lung cancer cells

机译：Caveolin-1调节紫杉醇诱导的多药耐药A549肺癌细胞的凋亡和侵袭能力

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摘要

The aim of the study was to investigate the effect and potential mechanism of caveolin-1 (Cav1) knockdown in paclitaxel-resistant lung cancer A549/Taxol cells. The human paclitaxel-resistant lung cancer cell line A549/Taxol was transfected with a Cav1 shRNA lentiviral vector. Interference efficiency for Cav1 was detected by real-time PCR and Western blotting. A MTT assay was used to determine cell proliferation, and flow cytometry was used to detect the cell cycle stage and apoptosis. Cell migration and invasion capability were detected by a transwell assay. Protein levels of related signaling molecules were detected by Western blotting. We successfully constructed a stable A549/Taxol cell line expressing low levels of Cav1. Cav1 knockdown significantly inhibited cell proliferation and induced G0/G1 arrest and cell apoptosis in vitro and in vivo. In addition, these effects correlated significantly with a reduction in cyclin D1 expression and activation of the Bcl-2/Bax-mediated mitochondrial apoptosis pathway. Furthermore, knockdown of Cav1 inhibited cell migration and invasion, and this may be related to the inhibition of AKT and the subsequent decreased protein expression of MMP2, MMP7 and MMP9.
机译：该研究的目的是研究caveolin-1（Cav1）敲低对紫杉醇耐药的肺癌A549 / Taxol细胞的作用及其潜在机制。用Cav1 shRNA慢病毒载体转染抗紫杉醇的人肺癌细胞株A549 / Taxol。通过实时PCR和Western印迹检测Cav1的干扰效率。使用MTT测定法测定细胞增殖，并使用流式细胞术检测细胞周期阶段和凋亡。通过transwell测定法检测细胞迁移和侵袭能力。通过Western印迹检测相关信号分子的蛋白质水平。我们成功构建了稳定的表达低水平Cav1的A549 / Taxol细胞系。 Cav1敲低显着抑制细胞增殖，并在体外和体内诱导G0 / G1阻滞和细胞凋亡。此外，这些影响与细胞周期蛋白D1表达的减少和Bcl-2 / Bax介导的线粒体凋亡途径的激活显着相关。此外，敲低Cav1抑制细胞迁移和侵袭，这可能与AKT的抑制以及随后MMP2，MMP7和MMP9蛋白表达的降低有关。

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