Nano-composite Carbon Pate Electrode Used for Biophysical Study of Ho3+ Ion Interaction with Human Serum Albumin V

M. R. Ganjali; F. Faridbod; A. Divsalar; A. A. Saboury; P. Norouzi; G. Rezaei Behbehani; S. Abdolahzadeh

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Nano-composite Carbon Pate Electrode Used for Biophysical Study of Ho3+ Ion Interaction with Human Serum Albumin V

机译：纳米复合碳酸盐电极用于Ho 3 + 离子与人血清白蛋白V相互作用的生物物理研究

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In this study, for the first time the interaction of Ho ion and human serum albumin (HSA) wasinvestigated in vitro by nano-composite carbon paste potentiometric sensor, fluorescence, andisothermal titration calorimetry at 27 and 37 C in biological pH. The resulted data from these methodsconfirm each other and they reveal that interaction of HSA with Ho ion showed a set of two bindingsites with negative cooperativity. Thermodynamic parameters for the interaction of holmium ions andHSA indicate that the concentration of this ion causes conformational changes on the structure of HSAwhich increasing the number of hydrophobic contacts of HSA.

机译：在这项研究中，首次通过纳米复合碳糊电位传感器，荧光和等温滴定热法在生物pH值下于27和37℃体外研究了Ho离子与人血清白蛋白（HSA）的相互作用。这些方法得到的数据相互证实，他们揭示了HSA与Ho离子的相互作用显示出两个结合位点，且具有负协同作用。 dynamic离子与HSA相互作用的热力学参数表明，该离子的浓度引起HSA结构的构象变化，从而增加了HSA的疏水接触数。

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《International Journal of Electrochemical Science》 |2010年第6期|共15页
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M. R. Ganjali; F. Faridbod; A. Divsalar; A. A. Saboury; P. Norouzi; G. Rezaei Behbehani; S. Abdolahzadeh;
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Nano-composite Carbon Pate Electrode Used for Biophysical Study of Ho3+ Ion Interaction with Human Serum Albumin V

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