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Antioxidant Study and Electroanalytical Investigation of Selected Herbal Samples Used in Folk Medicine

机译:精选中草药样品的抗氧化研究和电分析研究

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The growing concern regarding oxidative-related cell damage has guided a myriad of investigationsconcerning the applicability of herbal mater to mop up reactive oxygen species generation. In this sense,the consumption of functional foods and the use of plants with antioxidant capacity are well understoodin folk medicine. Thus, this work was intended to electroanalytically study tea and tincture preparationsof selected herbs used in folk medicine in order to better understand their redox features. Henceforth,samples of Cinnamomum zeylanicum (CZB), Pterodon emarginatus (PER) (PES), Cotyledon orbiculata(COL), Syzygium aromaticum (SAF), Commiphora myrrha (CML), Cyrtopodium puntactum (CPR),Uncaria tomentosa (UTR) and Brosimum gaudichaudii (BGR) were also analyzed in colorimetric testsof radical scavenging activity ABTS and DPPH. Differential pulse voltammetry (DPV) measurementswere performed using a potentiostat/galvanostat. A carbon paste electrode, Pt wire and theAg/AgCl/KClsat 3 M were used as working, counter and reference electrodes, respectively. The resultsevidenced that CZB, PER, SAF, CML and BGR presented better antioxidant features in a tincture form,probably due to better aglycone solubilization in hydroethanolic solutions, while CPR and UTRpresented better antioxidant activity in aqueous preparations because of the polyphenolic components.The highest observed peak amongst all analyzed samples was that of SAF, attesting to its high contentof electroactive compounds, while BGR, COL and PER showed good distribution of all anodic peak,being all correlated to phenolic metabolytes. Therefore, the antioxidant activity of these preparationschanges heavily according to the employed extraction fluids, and this activity can be assessed either byelectrochemical or colorimetric techniques, henceforth rendering reproducible results.
机译:关于与氧化有关的细胞损伤的日益增长的关注已经引导了许多有关草药用于清除活性氧产生的研究。从这个意义上说,民间食品对功能食品的消费和具​​有抗氧化能力的植物的使用已广为人知。因此,这项工作旨在对在民间医学中使用的精选草药的茶和tin制剂进行电分析研究,以便更好地了解其氧化还原特性。从此以后,样品包括:桂皮(CZB),翡翠翼龙(PER)(PES),子叶子叶(COL),芳族丁香(SAF),没药Commonphora myrrha(CML),蓬蓬石蒜(Cyrtopodium Puntactum(CPR)),刺桐(Uncaria tomentosa)还通过比色法检测自由基清除活性ABTS和DPPH来分析高迪高迪(BGR)。使用恒电位仪/恒电流仪进行差分脉冲伏安法(DPV)测量。碳糊电极,Pt线和Ag / AgCl / KClsat 3 M分别用作工作电极,对电极和参比电极。结果表明,CZB,PER,SAF,CML和BGR以cture剂形式表现出更好的抗氧化特性,这可能是由于氢乙醇溶液中糖苷配体的溶解性更好,而CPR和UTR在水性制剂中由于多酚成分表现出了更好的抗氧化活性。在所有分析样品中,SAF的峰均为SAF,表明其含有较高的电活性化合物,而BGR,COL和PER则显示了所有阳极峰的良好分布,均与酚类代谢产物相关。因此,这些制剂的抗氧化活性根据所使用的提取液而有很大的变化,并且该活性可以通过电化学或比色技术进行评估,从而提供可再现的结果。

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